CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 9717 > Article
Presentation
27 April 2016 Adaptive optics without guide stars (Conference Presentation)
Jerome Mertz, Jiang Li, Devin Beaulieu, Hari P. Paudel, Roman Barankov, Thomas G. Bifano
Author Affiliations +
Proceedings Volume 9717, Adaptive Optics and Wavefront Control for Biological Systems II; 97170F (2016) https://doi.org/10.1117/12.2218574
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
Adaptive optics is a strategy to compensate for sample-induced aberrations in microscopy applications. Generally, it requires the presence of "guide stars" in the sample to serve as localized reference targets. We describe an implementation of conjugate adaptive optics that is amenable to widefield (i.e. non-scanning) microscopy, and can provide aberration corrections over potentially large fields of view without the use of guide stars. A unique feature of our implementation is that it is based on wavefront sensing with a single-shot partitioned-aperture sensor that provides large dynamic range compatible with extended samples. Combined information provided by this sensor and the imaging camera enable robust image de-blurring based on a rapid estimation of sample and aberrations obtained by closed-loop feedback. We present the theoretical principle of our technique and experimental demonstrations using both trans-illumination and fluorescence microscopes. Finally, we apply our technique to mouse brain imaging.
Conference Presentation
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Jerome Mertz, Jiang Li, Devin Beaulieu, Hari P. Paudel, Roman Barankov, and Thomas G. Bifano "Adaptive optics without guide stars (Conference Presentation)", Proc. SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, 97170F (27 April 2016); https://doi.org/10.1117/12.2218574
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Adaptive optics
Stars
Image sensors
Microscopy
Sensors
Aberration correction
Cameras
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top