CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC11960 > Article
Presentation
7 March 2022 Ultraviolet scattering and photoacoustic remote sensing (sPARS) microscopy for virtual H and E histology
Nathaniel J. M. Haven, Matthew T. Martell, Brendyn D. Cikaluk, Brendon S. Restall, Ewan McAlister, Roger J. Zemp, Sveta Silverman, Lashan Peiris, Jean Deschenes, Xingyu Li
Author Affiliations +
Proceedings Volume PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022; PC1196005 (2022) https://doi.org/10.1117/12.2610317
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Currently, there is an inability to obtain fast realistic label-free virtual histopathological images of tissues. We previously introduced ultraviolet photoacoustic remote sensing microscopy as a method to obtain virtual hematoxylin contrast albeit without the ability to obtain virtual eosin contrast. By utilizing UV scattering as a high-resolution eosin channel we are able to produce complete H&E-like virtual histology of unstained human breast lumpectomy specimen sections. By further leveraging a novel colormap matching algorithm with this UV scattering, we generate H&E-like output that is shown to have strong concordance with true H&E-stained adjacent sections, showing promising diagnostic utility.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Nathaniel J. M. Haven, Matthew T. Martell, Brendyn D. Cikaluk, Brendon S. Restall, Ewan McAlister, Roger J. Zemp, Sveta Silverman, Lashan Peiris, Jean Deschenes, and Xingyu Li "Ultraviolet scattering and photoacoustic remote sensing (sPARS) microscopy for virtual H and E histology", Proc. SPIE PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022, PC1196005 (7 March 2022); https://doi.org/10.1117/12.2610317
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Scattering
Ultraviolet radiation
Microscopy
Photoacoustic spectroscopy
Remote sensing
Gold
Laser scattering
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top