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17 March 2023 In-vivo optophysiology in rodent eyes using phase-sensitive optical coherence tomography
Bingyao Tan, Huakun Li, Veluchamy A. Barathi, Leopold Schmetterer, Tong Ling
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Proceedings Volume PC12360, Ophthalmic Technologies XXXIII; PC123600A (2023) https://doi.org/10.1117/12.2650161
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
In recent years, there have been vast interests in probing photoreceptor dynamics using optical coherence tomography (OCT). Most of the successful demonstrations implemented adaptive optics or digital adaptive optics to resolve individual cones or rods in human subjects. Here we use phase information to trace the photoreceptor response in rodents using an ultrahigh-resolution, phase-sensitive, spectral-domain OCT. As a result, two types of nanoscale signals (monophasic and biphasic) were detected with a clear separation in depth. The monophasic signal is less susceptible to stimulus intensity and saturated from a 3% breach rate.
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Bingyao Tan, Huakun Li, Veluchamy A. Barathi, Leopold Schmetterer, and Tong Ling "In-vivo optophysiology in rodent eyes using phase-sensitive optical coherence tomography", Proc. SPIE PC12360, Ophthalmic Technologies XXXIII, PC123600A (17 March 2023); https://doi.org/10.1117/12.2650161
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KEYWORDS
Optical coherence tomography
Adaptive optics
In vivo imaging
Animal model studies
Head
Human subjects
Optical signal processing
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