Easy and reliable way to perform single molecule FRET measurements (Conference Presentation)

Felix Koberling; Marcelle König; Maria Loidolt-Krüger; Fabio Barachati; Matthias Patting; Marcus Sackrow; Kamil Bobowski; Mathias Bayer; Rainer Erdmann

doi:10.1117/12.2649871

15 March 2023 Easy and reliable way to perform single molecule FRET measurements (Conference Presentation)

Felix Koberling, Marcelle König, Maria Loidolt-Krüger, Fabio Barachati, Matthias Patting, Marcus Sackrow, Kamil Bobowski, Mathias Bayer, Rainer Erdmann

Author Affiliations +

Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC1238603 (2023) https://doi.org/10.1117/12.2649871
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Luminosa, the new single photon counting confocal microscope from PicoQuant addresses the challenges of an expanding single-molecule FRET community. In this talk we present how easily these measurements can be performed with Luminosa single photon-counting confocal microscope and how all necessary correction parameters are automatically determined requiring no interaction from the user by employing methodologies benchmarked by the scientific community. We will also show how the variable PSF feature can be used in such measurements to fine-tune the observation window of freely diffusing biomolecules.

Conference Presentation

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Felix Koberling, Marcelle König, Maria Loidolt-Krüger, Fabio Barachati, Matthias Patting, Marcus Sackrow, Kamil Bobowski, Mathias Bayer, and Rainer Erdmann "Easy and reliable way to perform single molecule FRET measurements (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC1238603 (15 March 2023); https://doi.org/10.1117/12.2649871

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KEYWORDS

Molecules

Fluorescence resonance energy transfer

Proteins

Confocal microscopy

Microscopes

Photonic microstructures

Point spread functions

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