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16 March 2023 Raman and SERS spectroscopy for EV characterization: detection of amyloid beta and bulk chemical analysis (Conference Presentation)
Meruyert Imanbekova, Tatu Rojalin, Silvia Hilt, Randy P. Carney, John C. Voss, Ayse Mine Saridag, Mehmet Kahraman, Humeyra Caglayan, Sebastian Wachsmann-Hogiu
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Proceedings Volume PC12394, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XX; PC1239406 (2023) https://doi.org/10.1117/12.2656344
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Extracellular vesicles (EVs) carry molecular cargo that includes nucleic acids, lipids, proteins, and various other biomarkers. Raman and SERS spectroscopy are label-free spectroscopy techniques based on inelastic scattering of laser light interacting with molecular vibrations. In our study, we employed Raman and SERS spectroscopy for the detection of amyloid beta protein in the molecular cargo of small EVs and bulk chemical analysis of EVs. We observed considerable variation as a reflection of the biochemical content of EVs related to the Aβ peptide incorporated in EVs extracted from the AD cell culture model. Next, we developed a new CMOS-based sensing platform for trapping, imaging, and chemical characterization of EVs via SERS (CMOS TrICC) with the experimental enhancement factor 5.0 × 104. We employed this platform for parallel trapping and sensitive biochemical analysis of the 100 nm nanospheres and EVs.
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Meruyert Imanbekova, Tatu Rojalin, Silvia Hilt, Randy P. Carney, John C. Voss, Ayse Mine Saridag, Mehmet Kahraman, Humeyra Caglayan, and Sebastian Wachsmann-Hogiu "Raman and SERS spectroscopy for EV characterization: detection of amyloid beta and bulk chemical analysis (Conference Presentation)", Proc. SPIE PC12394, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XX, PC1239406 (16 March 2023); https://doi.org/10.1117/12.2656344
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KEYWORDS
Raman spectroscopy
Surface enhanced Raman spectroscopy
Chemical analysis
Proteins
Imaging spectroscopy
Laser scattering
Light scattering
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