Visualization of cellular membrane charging induced by MHz compressed trains of nanosecond pulsed electric fields

Mark A. Keppler; Ines Abrahamsson; Gleb P. Tolstykh; Benjamin M. Kasukonis; Vladislav V. Yakovlev; Joel N. Bixler

doi:10.1117/12.3021425

13 March 2024 Visualization of cellular membrane charging induced by MHz compressed trains of nanosecond pulsed electric fields

Mark A. Keppler, Ines Abrahamsson, Gleb P. Tolstykh, Benjamin M. Kasukonis, Vladislav V. Yakovlev, Joel N. Bixler

Author Affiliations +

Proceedings Volume PC12840, Optical Interactions with Tissue and Cells XXXV; PC1284002 (2024) https://doi.org/10.1117/12.3021425
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Nanosecond electric pulses (nsEP) are effective in biomedical applications like cancer treatment, gene therapy, and drug delivery due to their ability to influence cellular membranes and intracellular processes without thermal damage. However, the high electric fields required for these bioeffects present challenges. Recent studies suggest that MHz compression of nsEP could lead to similar effects at lower field strengths, enhancing safety and efficacy. This study leverages streak camera and optical streaking microscopy to examine membrane charging dynamics from nsEP bursts. Results will broaden understanding of membrane responses to nsEP, potentially improving their effectiveness and safety in biomedical applications.

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Citation Download Citation

Mark A. Keppler, Ines Abrahamsson, Gleb P. Tolstykh, Benjamin M. Kasukonis, Vladislav V. Yakovlev, and Joel N. Bixler "Visualization of cellular membrane charging induced by MHz compressed trains of nanosecond pulsed electric fields", Proc. SPIE PC12840, Optical Interactions with Tissue and Cells XXXV, PC1284002 (13 March 2024); https://doi.org/10.1117/12.3021425

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