Photoacoustic remote sensing microscopy and meta-LASE microscopy for visualizing virtual histology, circulating tumor cells, and tissue energetics

Pradyumna Kedarisetti; Nathaniel J. M. Haven; Matthew T. Martell; Frank R. Wuest; Roger J. Zemp

doi:10.1117/12.3002145

13 March 2024 Photoacoustic remote sensing microscopy and meta-LASE microscopy for visualizing virtual histology, circulating tumor cells, and tissue energetics

Pradyumna Kedarisetti, Nathaniel J. M. Haven, Matthew T. Martell, Frank R. Wuest, Roger J. Zemp

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Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540I (2024) https://doi.org/10.1117/12.3002145
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The detection and quantification of circulating tumor cells (CTCs) in a patient’s bloodstream is a promising minimally invasive avenue for improving cancer diagnostics and prognostics. However, clinical utilization of CTC-based diagnostics has been limited due to the arduous nature of currently employed CTC detection strategies which largely involve tedious staining and purification procedures. We report the use of photoacoustic remote sensing microscopy to enable the label-free visualization of peripheral blood mononuclear cells for the purpose of categorizes white blood cell populations and identifying putative circulating tumor cells present in the blood stream. Image analysis of cellular characteristics is performed, and identification of cell phenotype is validated by comparison to histological staining.

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Pradyumna Kedarisetti, Nathaniel J. M. Haven, Matthew T. Martell, Frank R. Wuest, and Roger J. Zemp "Photoacoustic remote sensing microscopy and meta-LASE microscopy for visualizing virtual histology, circulating tumor cells, and tissue energetics", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540I (13 March 2024); https://doi.org/10.1117/12.3002145

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KEYWORDS

Biological imaging

Photoacoustic spectroscopy

Remote sensing

Tumors

White blood cells

Diagnostics

Photoacoustic imaging

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