Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

Soren D. Konecky; Tyler B. Rice; Bruce J. Tromberg; Chris M.. Owen; Pablo A. Valdes; David W. Roberts; Kolbein Kolste; Frederic Leblond; Keith D. Paulsen; Brian C. Wilson

doi:10.1117/1.JBO.17.5.056008

4 May 2012 Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models

Soren D. Konecky, Tyler B. Rice, Bruce J. Tromberg, Chris M.. Owen, Pablo A. Valdes, David W. Roberts, Kolbein Kolste, Frederic Leblond, Keith D. Paulsen, Brian C. Wilson

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 5, 056008 (May 2012). https://doi.org/10.1117/1.JBO.17.5.056008

Abstract

Multifrequency (0 to 0.3 mm−1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼ 1.5 in reduced scattering coefficient for all wavelengths and was ∼ 1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.

Citation Download Citation

Soren D. Konecky, Tyler B. Rice, Bruce J. Tromberg, Chris M.. Owen, Pablo A. Valdes, David W. Roberts, Kolbein Kolste, Frederic Leblond, Keith D. Paulsen, and Brian C. Wilson "Spatial frequency domain tomography of protoporphyrin IX fluorescence in preclinical glioma models," Journal of Biomedical Optics 17(5), 056008 (4 May 2012). https://doi.org/10.1117/1.JBO.17.5.056008

Published: 4 May 2012

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY