In vivo skin optical clearing efficacy quantification of clinically compatible agents using line-field confocal optical coherence tomography

Sergey M. Zaytsev; Marine Amouroux; Valery V. Tuchin; Elina A. Genina; Walter Blondel

doi:10.1117/1.JBO.28.5.055002

27 May 2023 In vivo skin optical clearing efficacy quantification of clinically compatible agents using line-field confocal optical coherence tomography

Sergey M. Zaytsev, Marine Amouroux, Valery V. Tuchin, Elina A. Genina, Walter Blondel

Author Affiliations +

Journal of Biomedical Optics, Vol. 28, Issue 5, 055002 (May 2023). https://doi.org/10.1117/1.JBO.28.5.055002

Abstract

Significance

The clinical use of optical methods for in vivo skin imaging is limited by skin strong scattering properties, which reduce image contrast and probing depth. The efficiency of optical methods can be improved by optical clearing (OC). However, for the use of OC agents (OCAs) in a clinical setting, compliance with acceptable non-toxic concentrations is required.

Aim

OC of in vivo human skin, combined with physical and chemical methods to enhance skin permeability to OCAs, was performed to determine the clearing-effectiveness of biocompatible OCAs using line-field confocal optical coherence tomography (LC-OCT) imaging.

Approach

Nine types of OCAs mixtures were used in association with dermabrasion and sonophoresis for OC protocol on three volunteers hand skin. From 3D images obtained every 5 min for 40 min, the intensity and contrast parameters were extracted to assess their changes during the clearing process and evaluate each OCAs mixture’s clearing efficacy.

Results

The LC-OCT images average intensity and contrast increased over the entire skin depth with all OCAs. The best image contrast and intensity improvement was observed using the polyethylene glycol, oleic acid, and propylene glycol mixture.

Conclusions

Complex OCAs featuring reduced component concentrations that meet drug regulation-established biocompatibility requirements were developed and proved to induce significant skin tissues clearing. By allowing deeper observations and higher contrast, such OCAs in combination with physical and chemical permeation enhancers may improve LC-OCT diagnostic efficacy.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Sergey M. Zaytsev, Marine Amouroux, Valery V. Tuchin, Elina A. Genina, and Walter Blondel "In vivo skin optical clearing efficacy quantification of clinically compatible agents using line-field confocal optical coherence tomography," Journal of Biomedical Optics 28(5), 055002 (27 May 2023). https://doi.org/10.1117/1.JBO.28.5.055002

Received: 2 December 2022; Accepted: 27 March 2023; Published: 27 May 2023

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
15 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY