Prof. Antoine Delon Profile

Prof. Antoine Delon

Professor at Lab Interdisciplinaire de Physique

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Publications (9)

Proceedings Article | 7 December 2021 Paper

Photobleaching and correlation spectroscopy for in-situ quantification of multi-labelled molecules on surfaces

Julius Sefkow-Werner, Irene Wang, Catherine Picart, Elisa Migliorini, Antoine Delon

Proceedings Volume 11922, 119220F (2021) https://doi.org/10.1117/12.2615697

KEYWORDS: Molecules, Fluorescence correlation spectroscopy, Spectroscopy, Molecular spectroscopy, Microscopes, Confocal microscopy, Signal detection, Microscopy, Glasses, Spectroscopic ellipsometry

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Proceedings Article | 7 April 2020 Presentation

Fluorescence speckle image correlation spectroscopy (Conference Presentation)

Anirban Sarkar, Irène Wang, Aditya Katti, Jörg Enderlein, Jacques Derouard, Antoine Delon

Proceedings Volume 11351, 1135108 (2020) https://doi.org/10.1117/12.2558129

KEYWORDS: Speckle, Scattering, Fluorescence correlation spectroscopy, Spectroscopy, Light scattering, Luminescence, Fluorescence spectroscopy, Imaging spectroscopy, Particles, Speckle pattern

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Fluorescence correlation spectroscopy is used extensively for quantitative characterization of biomolecules at very low concentration. However, light aberration and scattering from the tissues are two major factors that affect the results strongly. Although adaptive optics arrangement can correct the aberrations of light to some extent, it fails completely to eliminate the light scattering effect. Recently, exploiting the fact that autocorrelation of a speckle pattern is a sharply peaked point spread function and the optical memory effect, non-invasive imaging of fluorescent sample through a scattering medium has been possible. However, it is also very challenging to measure the dynamic properties of the fluorescent molecules or particles through a scattering layer due to poor signal to noise ratio. In this study, we employ a modality based on speckle cross-correlation enabled via optical memory effect to study two dimensional (2D) diffusion of fluorescent particles hidden behind a scattering film. We realized a 2D diffusing model system by confining fluorescent polystyrene beads of 1µm diameter at the water/air interface behind a TiO2 diffuser. The experimental set up was built up in an epifluorescence configuration. The fluorescent beads were excited by an illumination speckle generated by the incident light in a plane-wave geometry while passing through the disordered TiO2 film. Similarly, the emitted fluorescent signal also traversed through the same TiO2 film to generate the detection speckle, which was eventually recorded by a high frame rate CMOS camera. The experimental set up has also been modelled numerically, where speckle pattern has been generated by a spherical wave, transmitted through a scattering object in an optical microscope. Moreover, the dependence of the speckle size on the numerical aperture, magnification, and the distance of the focal plane from the bead plane has also been studied. The numerical results have been compared with the experimental values to estimate the speckle size. Furthermore, we have evaluated the 2D diffusion constant by monitoring the widening of the 2D speckle cross-correlation function versus lag time. This result has been compared with that obtained with the single particle tracking method without the scattering layer. Quantitative agreement between the results obtained by the speckle cross-correlations and the single particle tracking technique without the diffuser establishes the potential application of this technique in correlation spectroscopy. Superimposed multiple beads speckle patterns were also studied and the results will be presented in the conference.

Proceedings Article | 6 April 2016 Presentation + Paper

Whole slide imaging of unstained tissue using lensfree microscopy

Sophie Nhu An Morel, Lionel Hervé, Thomas Bordy, Olivier Cioni, Antoine Delon, Catherine Fromentin, Jean-Marc Dinten, Cédric Allier

Proceedings Volume 9711, 97111L (2016) https://doi.org/10.1117/12.2210831

KEYWORDS: Tissues, Holograms, Digital imaging, Microscopy, Pathology, Holography, RGB color model, Sensors, Colon, Biopsy

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Proceedings Article | 14 July 2015 Paper

Wide-field lensfree imaging of tissue slides

Sophie Nhu An Morel, Antoine Delon, Pierre Blandin, Thomas Bordy, Olivier Cioni, Lionel Hervé, Catherine Fromentin, Jean-Marc Dinten, Cédric Allier

Proceedings Volume 9536, 95360K (2015) https://doi.org/10.1117/12.2183462

KEYWORDS: Tissues, Holograms, Microscopes, Sensors, Reconstruction algorithms, Image retrieval, 3D image reconstruction, Image resolution, Biopsy, Optical filters

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Proceedings Article | 7 March 2014 Paper

Accuracy of adaptive optics correction using fluorescence fluctuations

Joseph Gallagher, Charles-Edouard Leroux, Irène Wang, Antoine Delon

Proceedings Volume 8978, 89780A (2014) https://doi.org/10.1117/12.2040428

KEYWORDS: Signal to noise ratio, Molecules, Luminescence, Diffusion, Adaptive optics, Aberration correction, Deformable mirrors, Monochromatic aberrations, Mirrors, Fluorescence correlation spectroscopy

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Proceedings Article | 4 March 2014 Paper

Determination of protein concentration on substrates using fluorescence fluctuation microscopy

Richard De Mets, Irène Wang, Joseph Gallagher, Olivier Destaing, Martial Balland, Antoine Delon

Proceedings Volume 8950, 895007 (2014) https://doi.org/10.1117/12.2040355

KEYWORDS: Molecules, Luminescence, Fluorescence correlation spectroscopy, Proteins, Diffusion, Microscopy, Confocal microscopy, Imaging spectroscopy, Solids, Spectroscopy

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Proceedings Article | 9 June 2011 Paper

Automatic laser alignment for multifocal microscopy using a LCOS SLM and a 32×32 pixel CMOS SPAD array

David Tyndall, Richard Walker, Krzysztof Nguyen, Rémi Galland, Jie Gao, Irène Wang, Meike Kloster, Antoine Delon, Robert Henderson

Proceedings Volume 8086, 80860S (2011) https://doi.org/10.1117/12.889738

KEYWORDS: Spatial light modulators, Sensors, Confocal microscopy, Microscopy, Photon counting, Detector arrays, Fluorescence correlation spectroscopy, Fluorescence lifetime imaging, CMOS sensors, Alignment procedures

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Proceedings Article | 14 April 2006 Paper

Spatial fluorescence cross-correlation spectroscopy between core and ring pinholes

Yoann Blancquaert, Antoine Delon, Jacques Derouard, Rodolphe Jaffiol

Proceedings Volume 6191, 61910B (2006) https://doi.org/10.1117/12.662244

KEYWORDS: Diffusion, Luminescence, Fluorescence correlation spectroscopy, Bioalcohols, Fluorescence spectroscopy, Spectroscopy, Molecules, Confocal microscopy, Optical fibers, Water

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Proceedings Article | 14 April 2006 Paper

Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates

Jacques Derouard, Antoine Delon, Rodolphe Jaffiol, Cyrille Vézy

Proceedings Volume 6191, 61910C (2006) https://doi.org/10.1117/12.662294

KEYWORDS: Luminescence, Molecules, Solids, Photon counting, Glasses, Statistical analysis, Molecular spectroscopy, Error analysis, Confocal microscopy, Particles

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Showing 5 of 9 publications

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