Dr. Anton E. Efimov Profile

Dr. Anton E. Efimov

at Shumakov Ins of Transplantology & Artificial Organ

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Publications (3)

Proceedings Article | 8 May 2014 Paper

Development and potential applications of microarrays based on fluorescent nanocrystal-encoded beads for multiplexed cancer diagnostics

Kristina Brazhnik, Regina Grinevich, Anton Efimov, Igor Nabiev, Alyona Sukhanova

Proceedings Volume 9129, 91292C (2014) https://doi.org/10.1117/12.2057825

KEYWORDS: Cancer, Proteins, Diagnostics, Silver, Flow cytometry, Prostate cancer, Luminescence, Multiplexing, Breast cancer, Molecules

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Advanced multiplexed assays have recently become an indispensable tool for clinical diagnostics. These techniques provide simultaneous quantitative determination of multiple biomolecules in a single sample quickly and accurately. The development of multiplex suspension arrays is currently of particular interest for clinical applications. Optical encoding of microparticles is the most available and easy-to-use technique. This technology uses fluorophores incorporated into microbeads to obtain individual optical codes. Fluorophore-encoded beads can be rapidly analyzed using classical flow cytometry or microfluidic techniques. We have developed a new generation of highly sensitive and specific diagnostic systems for detection of cancer antigens in human serum samples based on microbeads encoded with fluorescent quantum dots (QDs). The designed suspension microarray system was validated for quantitative detection of (1) free and total prostate specific antigen (PSA) in the serum of patients with prostate cancer and (2) carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA 15-3) in the serum of patients with breast cancer. The serum samples from healthy donors were used as a control. The antigen detection is based on the formation of an immune complex of a specific capture antibody (Ab), a target antigen (Ag), and a detector Ab on the surface of the encoded particles. The capture Ab is bound to the polymer shell of microbeads via an adapter molecule, for example, protein A. Protein A binds a monoclonal Ab in a highly oriented manner due to specific interaction with the Fc-region of the Ab molecule. Each antigen can be recognized and detected due to a specific microbead population carrying the unique fluorescent code. 100 and 231 serum samples from patients with different stages of prostate cancer and breast cancer, respectively, and those from healthy donors were examined using the designed suspension system. The data were validated by comparing with the results of the “gold standard” enzyme-linked immunosorbent assay (ELISA). They have shown that our approach is a good alternative to the diagnostics of cancer markers using conventional assays, especially in early diagnostic applications.

Proceedings Article | 22 May 2013 Paper

High-resolution 3D structural and optical analyses of hybrid or composite materials by means of scanning probe microscopy combined with the ultramicrotome technique: an example of application to engineering of liquid crystals doped with fluorescent quantum dots

Konstantin Mochalov, Anton Efimov, Alexey Yu. Bobrovsky, Igor Agapov, Anton Chistyakov, Vladimir Oleinikov, Igor Nabiev

Proceedings Volume 8767, 876708 (2013) https://doi.org/10.1117/12.2017088

KEYWORDS: Luminescence, Liquid crystals, Atomic force microscopy, Matrices, Scanning probe microscopy, 3D metrology, Polymers, 3D modeling, 3D image reconstruction, Imaging spectroscopy

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Proceedings Article | 11 October 2012 Paper

Novel cholesteric materials doped with CdSe/ZnS quantum dots with photo- and electrotunable circularly polarized emission

Konstantin Mochalov, Alexey Yu. Bobrovsky, Vladimir Oleinikov, Alyona Sukhanova, Anton Efimov, Valery Shibaev, Igor Nabiev

Proceedings Volume 8475, 847514 (2012) https://doi.org/10.1117/12.929729

KEYWORDS: Luminescence, Liquid crystals, Ultraviolet radiation, Quantum dots, Polarization, Polymers, Photon polarization, Electro optics, Transmittance, 3D image reconstruction

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