The use of metallic nanoparticles in applications ranging from drug delivery to consumer electronics has exploded in the last two decades. Although this broad range of use cases has brought about technological revolutions in multiple fields, the effects of widespread production and subsequent human exposure to these nanoparticles have yet to be fully understood. New imaging techniques are a critical part of developing a more complete understanding of chronic exposure and biodistribution. Here we present a novel label free luminescence imaging technique to analyze the biodistribution, content, and biological context of metallic nanoparticles using multiphoton luminescence.
Profiling the heterogeneous landscape of cell types and biomolecules is rapidly being adopted to address current imperative research questions. Precision medicine seeks advancements in molecular spatial profiling techniques with highly multiplexed imaging capabilities and sub-cellular resolution, which remains an extremely complex task. Surface-enhanced Raman spectroscopy (SERS) imaging offers new promise through the utilization of nanoparticle-based contrast agents that exhibit narrow spectral features and molecular specificity.
Herein, we report the first demonstration of simultaneously multiplexing 26 different nanoparticles in a single imaging pixel with subcellular resolution.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.