Dr. Benjamin Hötzer Profile

Dr. Benjamin Hötzer

at DORUCON

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Publications (4)

Proceedings Article | 1 August 2017 Paper

Laser-induced stabilisation of the tympanic membrane

Sophie A. Schacht, Patricia Stahn, Marius Hinsberger, Benjamin Hoetzer, Bernhard Schick, Gentiana Wenzel

Proceedings Volume 10417, 104170Q (2017) https://doi.org/10.1117/12.2291758

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SPIE Journal Paper | 7 May 2013

Fluorescence-based determination of the copper concentration in drinking water

Benjamin Hötzer, Timo Scheu, Gregor Jung, Stefan Castritius

OE, Vol. 52, Issue 05, 053602, (May 2013) https://doi.org/10.1117/12.10.1117/1.OE.52.5.053602

KEYWORDS: Green fluorescent protein, Copper, Luminescence, Water, Absorption, Ions, Metals, Optical filters, Optical engineering, Resonance energy transfer

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Proceedings Article | 18 December 2012 Paper

Measurement of the copper concentration in drinking water based on changes of the fluorescence lifetime of the green fluorescent protein

Benjamin Hötzer, Timo Scheu, Gregor Jung, Stefan Castritius

Proceedings Volume 8550, 855021 (2012) https://doi.org/10.1117/12.981170

KEYWORDS: Green fluorescent protein, Copper, Luminescence, Absorption, Water, Ions, Metals, Optical filters, Fluorescence resonance energy transfer, Calibration

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Copper is a heavy metal, which is used in heat and electrical conductors and in a multitude of alloys in the technical context. Moreover, it is a trace element that is essential for the life of organisms but can cause toxic effects in elevated concentrations. Maximum limits in water and for beverages exist. Beyond that there is a need for the control of copper concentrations in the fields of sewage and agriculture. Hence, competitive measurement systems that allow for the fast, user-friendly and reliable detection are presumed to have a large potential market. One prominent class of naturally occurring fluorophores is the Green Fluorescent Protein (GFP). GFP originally stems from the jellyfish Aequorea Victoria and has found its way in various applications e.g. as biosensors in basic research and for monitoring gene expression. Exploiting GFP in plant cells allowed for the visualization of the copper uptake by changes in the GFP fluorescence. In principle changes in the fluorescence intensity or in the fluorescence lifetime can be utilized to determine copper concentrations. However, lifetime changes have the advantage of omitting calibration measurements and therefore make this method ideally suited for sensing purposes. Here the decrease of the fluorescence lifetime of GFP by Förster resonance energy transfer (FRET) is used to measure the copper ion concentration in drinking water. Therefore a system is developed that is based on a GFP sample in a predefined concentration. The GFP mutant can be excited with blue light. For binding of copper ions a His-tag is included in the GFP. After measuring the fluorescence lifetime of the pure GFP the copper determination of the sample is performed by lifetime measurement. Therefore the lifetime can be assigned to the copper concentration of the GFP-doped drinking water sample. In summary a method for the quantification of copper ions based on changes of the fluorescence lifetime of GFP is developed and the measurement of the copper concentration in water samples is performed.

Proceedings Article | 17 May 2007 Paper

Dye based light sensor for tag integration

I. Sayhan, B. Hoetzer, J. Woellenstein, E. Rose, Th. Becker

Proceedings Volume 6589, 65890A (2007) https://doi.org/10.1117/12.723758

KEYWORDS: Optical sensors, Polymers, Electrodes, Liquids, Polymeric sensors, Solar cells, Glasses, Molecules, Electrons, Transparent conductors

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