KEYWORDS: Optical coherence tomography, Gold, Nanoparticles, Silver, Ovarian cancer, Signal to noise ratio, Scattering, Light scattering, In vivo imaging, Nanorods
For OCT imaging, enhancing contrast efficiency will lead to significant improvements in the detection limits in cancer. Recently, noble metal nanoparticles are considered to be better contrast agents than traditional ones, especially for gold and silver. Silver nanoparticles have more attractive optical properties than gold nanoparticles. But they are employed far less because of its poor chemical stability. In this paper, we introduced our recent progress on a new application of using gold/silver alloy nanoparticles as OCT contrast agents in the detection of ovarian cancer. The scattering properties and sensitivity of silver were investigated. By means of tuning LSPR wavelengths of the nanoparticles, they were able to match the central wavelength of light used in OCT. Before carrying out animal experiments, we evaluated the different performances of alloy nanoparticles and gold nanorods in vitro. It has been sufficiently demonstrated that the alloy nanoparticles revealed stronger OCT signals than gold nanorods because of the better scattering properties. Then in vivo study, we compared the contrast enhancement of gold/silver alloy nanoparticles and gold nanorods on the ovarian cancer model mice. This study contributes a new kind of contrast agent in OCT imaging, which has a profound effect on drug delivery and further therapeutic action.
A unique, sensitive, and highly specific fluoroimmunoassay system for antigen detection using gold and quantum dot
nanoparticles has been developed. The assay is based on the fluorescence quenching of quantum dots caused by gold
nanoparticles coated with antibody. To demonstrate its analytical capabilities, the CdTe quantum dots were coated with
anti-HBsAg monoclonal antibodies (QDs-MAb1) and gold nanoparticles coated with another anti-HBsAg monoclonal
antibodies (GNPs-MAb2) which specifically bound with HBsAg could sandwich the HBsAg captured by the
immunoreactions. The sandwich-type immunocomplex was formed and the fluorescence intensity of quantum dots was
measured. The results showed that the fluorescence intensity of quantum dots at 570 nm was negative linear proportional
to the HBsAg concentration logarithm, and the limit of detection of the HBsAg was 0.928 ng/mL. This new system can
be extended to detect target molecules with matched antibodies and has broad potential applications in immunoassay and
disease diagnosis.
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