Our workgroup has recently developed a SPIM-based flow cytometer to detect fluorescent-labeled objects in whole blood [1]. It was used to visualize, detect, and magnetically separate fluorescent-labeled objects without hydrodynamic focusing. The flow cell holder, the magnet shifting system, and the camera filters system of the developed flow cytometer were printed on a standard FDM 3D printer. Here we have modified the optical system by a prism with a printed mounting system to minimize images' parasitic reflections and coma effect. We have also modified the magnetic concentrator system to make magnetic field distribution more efficient for magnetic separation and use a smaller permanent magnet in the system, thus adding whitelight source placement for the separation flow cell.
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