Making sense of the brain network and functions from individual neuronal activity is a challenging task. We developed a data analysis pipeline, combining unsupervised learning and supervised learning methods to reveal some of the intricacies of brain function in zebrafish. We were interested in particular to apply it to the senses of hearing, and balance. Using Optical Tweezers, we manipulated optically and individually each of the four ear stones which reside in the inner ear. Consequently, we simulated sound and acceleration in an alive zebrafish with laser beams. I will present the study of behaviour and neural responses to these stimulations.
Otoliths are calcium carbonate crystals located in fish ears. They play an important role in zebrafish for hearing, its sense of balance and acceleration. Many studies have been conducted to understand its structure, function but also development conditions. However the encoding in the brain as a movement sensor remains unknown. Here we developed a non-invasive system capable of manipulating one or two otoliths simultaneously in different directions to simulate movement or acceleration and sound. Our system uses optical traps created with an infra-red laser at different positions on the otoliths creating forces in chosen directions. However, as the optical traps need to go through brain tissue in a live fish, it becomes difficult to determine the exact forces applied. In this study we investigate the limits of forces determination. We will present the theory and experimental measurements of optical tweezers applied to otoliths which we mostly published in Nature Communications (doi:10.1038/s41467-017-00713-2). We will also present our latest result on brain imaging in response to artificial acceleration and sound.
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