Sensory perceptions entail the activation of thousands of interrelated neurons, each of these neurons can fire at distinct rates at different times. Despite this vast ‘activity space’ not all possible combinations of activity happen – instead lower dimensional subspaces define what are valid or invalid patterns. However, even the most advanced manipulation approaches so far are incapable of recreating a specific multi-neuron pattern. Here we describe a new approach based on holographic multiphoton optogenetics that allows us to write the numerically correct number of action potentials into a group of neurons, allowing us to write directly and flexibly to activity space.
Sensory perception involves the coordinated effort of thousands of intermingled neurons that act together in groups to amplify and strengthen perceptions. We developed a new approach to edit neural population codes in the cortex during behavior using spatiotemporally precise multiphoton optogenetics. These tools allow us to manipulate individual or groups of cells selected by their functional properties with the precision to write in particular trains of action potentials. We are using these tools to probe how different patterns of activity added to the mouse visual cortex change the overall firing patterns in the cortex, recruiting inhibition, and altering sensory representations.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.