Dr. Jerry Sebag Profile

Dr. Jerry Sebag

Vitreo-Retinal Surgeon at VMR Institute

SPIE Involvement:

Conference Program Committee | Author

Proceedings Article | 10 May 2023 Presentation + Paper

OCT velocimetry of vitreous dynamics induced by ocular saccades

Evangeline Priyadharshini Devaraj, Daniel Ruminski, Bartlomiej Kaluzny, J. Sebag, Ireneusz Grulkowski

Proceedings Volume PC12367, PC123670H (2023) https://doi.org/10.1117/12.2652861

KEYWORDS: Vitreous, In vivo imaging, Velocimetry, Optical coherence tomography, Eye, Prototyping, Particle image velocimetry, Optical tracking, Optical flow, Opacity

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Proceedings Article | 9 March 2020 Presentation

In vivo vitreous imaging using optical coherence tomography (Conference Presentation)

Daniel Ruminski, Ashish Gupta, Jerry Sebag, Silvestre Manzanera, Pablo Artal, Ireneusz Grulkowski

Proceedings Volume 11218, 112180G (2020) https://doi.org/10.1117/12.2545871

KEYWORDS: Vitreous, In vivo imaging, Coherence imaging, Optical imaging, Optical coherence tomography, Eye, Image segmentation, Tissue optics, Algorithm development, Visualization

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Proceedings Article | 13 March 2019 Presentation

In vivo imaging of vitreous opacities with full-eye-length SS-OCT (Conference Presentation)

Ireneusz Grulkowski, Ana Rodríguez-Aramendía, Silvestre Manzanera, Yiwei Chen, Juan Mompean, Fernando Díaz-Doutón, Jaume Pujol, J. Sebag, Pablo Artal

Proceedings Volume 10858, 108581B (2019) https://doi.org/10.1117/12.2509474

KEYWORDS: Vitreous, Opacity, In vivo imaging, Optical coherence tomography, Visualization, Transparency, Crystals, Eye, Optical imaging, Prototyping

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Proceedings Article | 16 May 2017 Presentation

Vitreous floaters (Conference Presentation)

K. Kershaw, Derek Nguyen, Kenneth Yee, Justin Nguyen, Michael Harrington, Jerry Sebag

Proceedings Volume 10045, 100450T (2017) https://doi.org/10.1117/12.2270412

KEYWORDS: Vitreous, Control systems, Particles, Proteins, Retina, Contrast sensitivity, Macula, Opacity, Light scattering, Dynamic light scattering

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BACKGROUND: Vitreous opacities and posterior vitreous detachment (PVD) disturb vision by degrading contrast sensitivity (AJO 172:7-12, 2016). Increased light scattering is the presumed mechanism. To test this hypothesis, dynamic light scattering (DLS) was performed on excised vitreous of patients with clinically significant floaters, and compared to macular pucker controls. METHODS: Undiluted, unfixed vitreous was procured during 25-gauge vitrectomy in 14 subjects (age = 59 ± 6.6 years) with clinically significant vitreous floaters, and 6 controls (age = 66.5 ± 8.7 years; P = 0.10) with macular pucker. Total protein concentration was determined by fluorescent Quant-iT^TM protein assay kit (Invitrogen/Molecular Probes, Eugene, OR) with bovine serum albumin (0500 ng/ml) as a standard. Fluorescence (excitation at 470 nm and emission at 570 nm) was measured using a Gemini XPS Dual-Scanning Microplate Spectrofluorometer and data analyzed using SoftMax Pro software (Molecular Devices, Sunnyvale, CA). DLS (NS300, Malvern Instruments, Westborough, MA) measurements were performed in each specimen after 10-fold dilution in phosphate buffered saline to optimize concentration in each specimen and determine the mean number of particles, the particle size distributions, and the average particle sizes. RESULTS: Total protein concentration in vitreous specimens trended higher in macular pucker controls (1037 ± 1038 μg/mL) than eyes with vitreous floaters (353.7 ± 141.1 μg/mL; P = 0.08). When normalized to total protein concentration, the number of particles in vitreous from floater eyes was more than 2-fold greater than controls (P < 0.04). Particle size distributions were similarly two-fold greater in vitreous from floater subjects as compared to controls (P < 0.05). The average particle size in vitreous from floater eyes was 315.8 ± 194.6 nm, compared to 147.7 ± 129.3 nm in macular pucker controls (P = 0.039). CONCLUSIONS: Vitreous from eyes with clinically significant floaters contains more particles of larger sizes as compared to controls, likely accounting for the degradation of contrast sensitivity previously found in these patients (Retina 34:1062-8, 2014; IOVS 56:1611–7, 2015; AJO 172:7-12, 2016). DLS could elucidate the underlying molecular abnormalities in patients afflicted with bothersome vitreous floaters and help develop clinical tools to better measure vitreous floaters as well as test the efficacy of various therapies.

Proceedings Article | 20 March 2015 Paper

Optical characterization of vitreous structure in health and disease

Ashwin Sampathkumar, Matin Khoshnevis, Jeffrey Ketterling, Jerry Sebag

Proceedings Volume 9307, 930714 (2015) https://doi.org/10.1117/12.2080543

KEYWORDS: Vitreous, Scattering, Gold, Light scattering, Opacity, Nanoparticles, Optical spheres, Spherical lenses, Absorption, Spectroscopy

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Patients with myopic vitreopathy (MV) and posterior vitreous detachment (PVD) see floaters, which often can degrade contrast sensitivity to a significant extent. The floaters are associated with irregularly shaped vitreous opacities. In contrast, asteroid hyalosis (AH), which is characterized by microscopic, spherical, white asteroid bodies (ABs) that move with vitreous displacement during eye movements, does not interfere significantly with vision. We hypothesize that the irregular surface of vitreous opacities associated with MV distinguish MV from AH and its smooth-surfaced ABs. A finite-element model was developed to characterize the light-scattering field of vitreous opacities in MV and AH. Vitreous opacities were modeled as spherical bodies and illuminated by a plane wave of light in the optical wavelength of 400-1000 nm. The model has provisions to add random perturbations to the spherical surfaces to vary light-scattering properties and mimic disturbances in vision from simple diffraction rings to more-complex patterns. Samples of ex vivo porcine vitreous (0.4-0.5 ml) were placed in a custom spectrophotometer and the static, light-scattering field of the sample was measured in the spectral range of 400-1000 nm with a resolution of 0.3 nm. Model solutions mimicking healthy vitreous and AH were experimentally validated using a laboratory optical apparatus. Model-based estimates of scattering cross-sections of calibrated gold nanoparticles were found to be in good agreement with experimental measurements. Simulation results potentially can complement experimental data to quantitatively characterize vitreous opacities and distinguish between structures that significantly impact vision, such as those due to myopic vitreopathy and aging, from those that have little impact, like ABs. Such techniques to determine the structural significance of vitreous opacification would be very useful in selecting patients for surgery as well as evaluating the efficacy of experimental therapies for floaters.

Proceedings Article | 7 March 2006 Paper

A new approach to evaluating the effects of pharmacologic vitreolysis on vitreous diffusion coefficients using dynamic light scattering

Rafat Ansari, Kwang Suh, Jerry Sebag

Proceedings Volume 6138, 61380B (2006) https://doi.org/10.1117/12.650731

KEYWORDS: Vitreous, Diffusion, Magnesium, Dynamic light scattering, Molecules, Particles, Surgery, In vitro testing, Collagen, Control systems

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SPIE Journal Paper | 1 January 2004 Open Access

Seeing the invisible: the challenge of imaging vitreous

Jerry Sebag

JBO, Vol. 9, Issue 01, (January 2004) https://doi.org/10.1117/12.10.1117/1.1627339

KEYWORDS: Vitreous, Optical fibers, Eye, Ultrasonography, Optical coherence tomography, Raman spectroscopy, Interfaces, Particles, Scanning laser ophthalmoscopy, In vitro testing

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SPIE Journal Paper | 1 January 2004 Open Access

Guest Editorial: Ophthalmic Diagnostics

Rafat Ansari, J. Sebag

JBO, Vol. 9, Issue 01, (January 2004) https://doi.org/10.1117/12.10.1117/1.1623762

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Proceedings Article | 7 June 2001 Paper

Celestial and terrestrial tele-ophthalmology: a health monitoring helmet for astronaut/cosmonaut and general public use

Rafat Ansari, Luigi Rovati, Jerry Sebag

Proceedings Volume 4245, (2001) https://doi.org/10.1117/12.429269

KEYWORDS: Eye, Raman spectroscopy, Atrial fibrillation, Tissues, Glucose, Blood circulation, Blood, Vitreous, Radiation effects, Laser Doppler velocimetry

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Proceedings Article | 7 June 2000 Paper

Preliminary findings using dynamic light scattering to study vitreous effects of pharmacologic vitreolysis

Jerry Sebag, N. Kitaya, Rafat Ansari, H. Karagoezian, Akitoshi Yoshida

Proceedings Volume 3908, (2000) https://doi.org/10.1117/12.387525

KEYWORDS: Vitreous, Diffusion, Collagen, Dynamic light scattering, Retina, Molecules, Particles, Eye, Light scattering, Eye models

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Proceedings Article | 23 June 1994 Paper

System to measure contrast-sensitivity function with a scanning-laser ophthalmoscope

Donald Frambach, Jerry Sebag, Terry Lawton, Alfredo Sadun

Proceedings Volume 2126, (1994) https://doi.org/10.1117/12.178550

KEYWORDS: Contrast sensitivity, Scanning laser ophthalmoscopy, Retina, Bragg cells, Spatial frequencies, Eye, Software development, Helium neon lasers, Scanning laser ophthalmoscopes, Reliability

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Proceedings Article | 24 June 1993 Paper

Raman spectroscopy characterization of diabetes effects on human vitreous in diabetic retinopathy

Jerry Sebag, Shuming Nie, Karen Reiser, Nai-Teng Yu

Proceedings Volume 1877, (1993) https://doi.org/10.1117/12.147541

KEYWORDS: Collagen, Vitreous, Raman spectroscopy, Bone, Surgery, Tissues, Fourier transforms, In vitro testing, Glucose, Spectroscopy

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Proceedings Article | 14 August 1992 Paper

Raman spectroscopy of human vitreous collagen in diabetic retinopathy

Jerry Sebag, Shuming Nie, Karen Reiser, Nai-Teng Yu

Proceedings Volume 1644, (1992) https://doi.org/10.1117/12.137422

KEYWORDS: Collagen, Vitreous, Raman spectroscopy, Spectroscopy, Surgery, In vitro testing, Tissues, Molecular spectroscopy, Biomedical optics, Luminescence

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Showing 5 of 13 publications

Conference Committee Involvement (23)

Ophthalmic Technologies XXXV

25 January 2025 | San Francisco, California, United States

Ophthalmic Technologies XXXIV

27 January 2024 | San Francisco, California, United States

Ophthalmic Technologies XXXIII

28 January 2023 | San Francisco, California, United States

Ophthalmic Technologies XXXII

22 January 2022 | San Francisco, California, United States

Ophthalmic Technologies XXXI

6 March 2021 | Online Only, California, United States

Ophthalmic Technologies XXX

1 February 2020 | San Francisco, California, United States

Ophthalmic Technologies XXIX

2 February 2019 | San Francisco, California, United States

Ophthalmic Technologies XXVIII

27 January 2018 | San Francisco, California, United States

Ophthalmic Technologies XXVII

28 January 2017 | San Francisco, California, United States

Ophthalmic Technologies XXVI

13 February 2016 | San Francisco, California, United States

Ophthalmic Technologies XXV

7 February 2015 | San Francisco, California, United States

Ophthalmic Technologies XXIV

1 February 2014 | San Francisco, California, United States

Ophthalmic Technologies XXIII

2 February 2013 | San Francisco, California, United States

Ophthalmic Technologies XXII

21 January 2012 | San Francisco, California, United States

Ophthalmic Technologies XXI

22 January 2011 | San Francisco, California, United States

Ophthalmic Technologies XX

23 January 2010 | San Francisco, California, United States

Ophthalmic Technologies XIX

24 January 2009 | San Jose, California, United States

Ophthalmic Technologies XVIII

19 January 2008 | San Jose, California, United States

Ophthalmic Technologies XVII

20 January 2007 | San Jose, California, United States

Ophthalmic Technologies XVI

21 January 2006 | San Jose, California, United States

Ophthalmic Technologies XV

22 January 2005 | San Jose, CA, United States

Ophthalmic Technologies XIV

24 January 2004 | San Jose, CA, United States

Ophthalmic Technologies XIII

25 January 2003 | San Jose, CA, United States

Showing 5 of 23 Conference Committees

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