Mr. Jesse R. Korber Profile

Jesse R. Korber

at Oregon Health & Science Univ

SPIE Involvement:

Author

Publications (5)

Proceedings Article | 20 April 2020 Presentation

Quantification of tissue distribution and therapeutic response using paired agent imaging in a chicken chorioallantoic membrane xenograft assay (Conference Presentation)

Kimberley Samkoe, Lei Wang, Jesse Korber, Summer Gibbs, Kenneth Tichauer

Proceedings Volume 11219, 112190E (2020) https://doi.org/10.1117/12.2547100

KEYWORDS: Tumors, Tissues, 3D modeling, Content addressable memory, In vivo imaging, Molecules, Statistical modeling

Read Abstract +

Proceedings Article | 19 February 2020 Presentation + Paper

Intracellular paired agent imaging enables improved evaluation of tyrosine kinase inhibitor target engagement

Allison Solanki, Lei Wang, Jesse Korber, Nathan McMahon, Kenneth Tichauer, Kimberley Samkoe, Summer Gibbs

Proceedings Volume 11219, 112190F (2020) https://doi.org/10.1117/12.2546234

KEYWORDS: Tumors, Tissues, Proteins, Imaging systems, In vivo imaging, Cancer, Visualization, Receptors

Read Abstract +

Targeting the aberrant epidermal growth factor receptor (EGFR) signaling pathway is an attractive choice for many cancers (e.g., non-small cell lung carcinoma (NSCLC) and head and neck squamous cell carcinoma (HNSCC)). Despite the development of promising therapeutics, incomplete target engagement and acquired resistance (e.g., mutagenesis and intracellular signaling pathway rewiring) ensure that curative options still elude patients. To address limitations posed by standard drug evaluation assays (e.g., western blot, bulk plasma monitoring, immunohistochemistry), we have developed a novel dynamic, fluorescence-based platform termed intracellular paired agent imaging (iPAI). iPAI quantifies intracellular protein target engagement using two matched small-molecule, cell membrane-permeable agents: one targeted to the protein of interest and one untargeted, which accounts for non-specific therapeutic uptake. Currently, our iPAI panel includes successfully characterized tyrosine kinase inhibitors targeting the kinase binding domain of numerous proteins in the EGFR pathway, including erlotinib (EGFR). Here, we present a pharmacokinetic uptake study using our novel iPAI erlotinib reagents: a targeted erlotinib probed conjugated to silicon tetramethylrhodamine (Erl- SiTMR-T) and an untargeted reagent conjugated to tetramethylrhodaime (Erl-TMR-UT). An initial uptake study in a cell derived xenograft (CDX) model of NSCLC was performed by administering the Erl iPAI reagents systemically via tail vein injection, where drug uptake was quantified in the tumor over time. Excitingly, evidence of heterogeneous uptake was observed in the iPAI injected cohort, displaying distinct drug-uptake within a single tumor. Characterization of additional iPAI agents targeting downstream effectors (e.g., AKT, PI3K, MEK and ERK) is ongoing and will allow us to visualize complex drug-target interactions and quantify their downstream signaling partners during treatment regimens for NSCLC and other cancers. Together, we anticipate these iPAI probes will improve understanding of current limitations in personalized cancer therapy.

Proceedings Article | 19 February 2020 Paper

Noninvasive in vivo mapping of intracellular signaling proteins using a pairing of targeted and untargeted fluorescently labeled small molecule kinase inhibitors

Kenneth Tichauer, Lei Wang, Allison Solanki, Jesse Korber, Summer Gibbs, Kimberley Samkoe

Proceedings Volume 11219, 112190K (2020) https://doi.org/10.1117/12.2545926

KEYWORDS: Information technology, Tissues, Molecules, In vivo imaging, Mathematical modeling, Imaging systems, Proteins, Cancer, Plasma, Differential equations

Read Abstract +

Proceedings Article | 28 February 2019 Paper

Simultaneous extracellular and intracellular quantification of EGFR using paired-agent imaging in an in ovo tumor model

Kimberley Samkoe, Emily Schultz, Allison Solanki, Lei Wang, Jesse Korber, Kenneth Tichauer, Summer Gibbs

Proceedings Volume 10859, 108590F (2019) https://doi.org/10.1117/12.2510778

KEYWORDS: Tumors, Content addressable memory, Luminescence, Proteins, Molecules, Tissues, Multispectral imaging, Receptors, Control systems, Tumor growth modeling

Read Abstract +

SPIE Journal Paper | 6 July 2018 Open Access

Nile Red derivatives enable improved ratiometric imaging for nerve-specific contrast

Jesse Korber, Connor Barth, Summer Gibbs

JBO, Vol. 23, Issue 07, 076002, (July 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.7.076002

KEYWORDS: Tissues, Nerve, Luminescence, Magnesium, In vivo imaging, Nitrogen, Quantum efficiency, Surgery, Molecules, Chromatography

Read Abstract +

DOWNLOAD PAPER

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years