Over the past few decades, a multitude of optical imaging techniques have emerged. Among them, full-field optical coherence tomography (FF-OCT) has gained significant importance in various biomedical applications. Indeed, FF-OCT stands out as a noninvasive and label-free imaging method capable of generating high-resolution 3D microscopic images of light-scattering biological specimens. However, FF-OCT approach is limited for in-vivo imaging and images from FF-OCT lack the specificity required for accurate diagnosis. Hence, there is a need to have access to in-vivo imaging and to incorporate additional contrast modalities, such as elastography, into the FF-OCT technique. Indeed, the combination of FF-OCT with shear wave elastography enables the quantitative assessment of tissue stiffness at a resolution of a few micrometers. In this context, we present a novel FF-OCT approach that enables single-shot acquisitions, making it well-suited for both in-vivo imaging and transient shear wave elastography.
The last few decades have seen the emergence of a huge number of optical imaging techniques. Among them, full-field optical coherence tomography (FF-OCT) has become valuable for many biomedical applications. Indeed, FF-OCT is a noninvasive and label-free imaging technique that produces high-resolution 3D microscopic images of scattering biological samples. However, FF-OCT images alone lack of specificity for accurate diagnosis. That is why it is necessary to add new contrast modalities to FF-OCT technique such as elastography. Indeed, coupling FF-OCT with shear wave elastography allows quantitative estimation of the stiffness at a resolution of a few micrometers. We present here our first results on coupling single-shot off-axis FF-OCT (SO-FF-OCT) method with transient shear wave elastography method.
The last few decades have seen the emergence of a huge number of optical imaging techniques. Among them, full-field optical coherence tomography (FF-OCT) has become valuable for many biomedical applications. Indeed, FF-OCT is a noninvasive and label-free imaging technique that produces high-resolution 3D microscopic images of scattering biological samples. Using FF-OCT approach for in-vivo imaging would enable the observation of cell-scale structures in living samples. Moreover, living samples have an active vascularization that can therefore be observed using Doppler imaging. We propose in this study a new FF-OCT approach that enables single-shot acquisitions which is suitable for in-vivo and Doppler imaging.
Paul Montgomery is a senior research scientist with the CNRS at the Engineering science, computer science and imaging laboratory (ICube) in Strasbourg, France. He has over 30 years' experience in developing optical instrumentation for the characterization of materials and biomaterials. He is currently interested in label-free far field nanoscopy, microsphere-assisted interference microscopy, local spectroscopy and the use of environmental chambers for measuring specific parameters. He is a senior member and a member of the Board of Directors of SPIE as well as being a member of IOP and SFO.
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