The study of micro-motion amplification technology has been widely concerned. Eulerian Video Magnification is one of the methods of concern and affirmation. Ischemia refers to the lack of blood flowing in certain parts of the body, which can occur in the limb, heart, brain or other parts of the body. Ischemia is a serious disease, which can cause serious tissue damage or let limbs not move. In this paper, we use Eulerian Video Magnification to initially detect whether someone suffers from limb ischemia by amplifying the movement of limb blood.
Gold nanoparticles are delivered into living cells by transient electroporation method to obtain intracellular surface-enhanced Raman spectroscopy (SERS). The subcellular localization of gold nanoparticles is characterized by transmission electron microscopy, and the forming large gold nanoaggregates are mostly found in the cytoplasm. The SERS detection of cells indicates that this kind of gold nanostructures induces a high signal enhancement of cellular chemical compositions, in addition to less cellular toxicity than that of silver nanoparticles. These results demonstrate that rapid incorporation of gold nanoparticles by electroporation into cells has great potential applications in the studies of cell biology and biomedicine.
Molecular characterization of semen that can be used to provide an objective diagnosis of semen quality is still lacking. Raman spectroscopy measures vibrational modes of molecules, thus can be utilized to characterize biological fluids. Here, we employed Raman spectroscopy to characterize and compare normal and abnormal semen samples in the fingerprint region (400-1800cm-1). Multivariate analysis methods including principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) were used for spectral analysis to differentiate between normal and abnormal semen samples. Compared with PCA-LDA analysis, PLS-DA improved the diagnostic results, showing a sensitivity of 77% and specificity of 73%. Furthermore, our preliminary quantitative analysis based on PLS algorithm demonstrated that spermatozoa concentration were relatively well predicted (R2=0.825). In conclusion, this study demonstrated that micro-Raman spectroscopy combined with multivariate methods can provide as a new diagnostic technique for semen analysis and differentiation between normal and abnormal semen samples.
Surface-enhanced Raman scattering (SERS) spectra of serum proteins purified from human serum samples were employed to detect colorectal cancer. Acetic acid as a new aggregating agent was introduced to increase the magnitude of the SERS enhancement. High-quality SERS spectra of serum proteins were acquired from 103 cancer patients and 103 healthy volunteers. Tentative assignments of SERS bands reflect that some specific biomolecular contents and protein secondary structures change with colorectal cancer progression. Principal component analysis combined with linear discriminant analysis was used to assess the capability of this approach for identifying colorectal cancer, yielding diagnostic accuracies of 100% (sensitivity: 100%; specificity: 100%) based on albumin SERS spectroscopy and 99.5% (sensitivity: 100%; specificity: 99%) based on globulin SERS spectroscopy, respectively. A partial least squares (PLS) approach was introduced to develop diagnostic models. An albumin PLS model successfully predicted the unidentified subjects with a diagnostic accuracy of 93.5% (sensitivity: 95.6%; specificity: 91.3%) and the globulin PLS model gave a diagnostic accuracy of 93.5% (sensitivity: 91.3%; specificity: 95.6%). These results suggest that serum protein SERS spectroscopy can be a sensitive and clinically powerful means for colorectal cancer detection.
In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ∼25°C ) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications.
Due to its high sensitivity, flexibility, and “fingerprints” sensing capability, Surface-enhanced Raman Spectroscopy
(SERS) is a very powerful method for characterization of substances. In this paper, two kinds of Radix Astragali with
different quality were firstly extracted through continuous circumfluence extraction method and then mixed with silver
nanoparticles for SERS detection. Most Raman bands obtained in Radix Astragali extraction solution are found at
300-7000cm-1 and 900-1390 cm-1. Although, major peak positions at 470, 556, 949, 1178 and 1286 cm-1 found in these
two kinds of Radix Astragali appear nearly the same, Raman bands of 556 and 1178 cm-1 are different in intensity, thus
may be used as a characteristic marker of Radix Astragali quality. In detail, we can make full use of the different
intensity of two different kinds but the same state at 556 cm-1 to describe the quality standard of Radix Astragali. Our
preliminary results show that SERS combining with continuous circumfluence extraction method may provide a direct,
accurate and rapid detection method of Radix Astragali.
The molecular characterization of ABO blood types, which is clinically significant in blood transfusion, has clinical and
anthropological importance. Polymerase chain reaction sequence-based typing (PCR-SBT) is one of the most commonly
used methods for the analysis of genetic bases of ABO blood types. However, such methods as PCR-SBT are
time-consuming and are high in demand of equipments and manipulative skill. Here we showed that membrane
electrophoresis based SERS method employed for studying the molecular bases of ABO blood types can provide rapidand
easy-operation with high sensitivity and specificity. The plasma proteins were firstly purified by membrane
electrophoresis and then mixed with silver nanoparticles to perform SERS detection. We use this method to classify
different blood types, including blood type A (n=13), blood type B (n=9) and blood type O (n=10). Combination of
principal component analysis (PCA) and liner discriminant analysis (LDA) was then performed on the SERS spectra of
purified albumin, showing good classification results among different blood types. Our experimental outcomes represent
a critical step towards the rapid, convenient and accurate identification of ABO blood types.
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