Hyper-Rayleigh Scattering (HRS) is a second-order nonlinear optical process in which the scattered light can be detected
at the second harmonic wavelength of the input laser beam. Due to its incoherent nature, it can be observed from metal
nanoparticles whose size is much smaller than the wavelength. Its sensitive response to the nanoparticle aggregation can
be used as a powerful diagnostic tool for the detection of biological target molecules. Many previous bio-sensing
applications of HRS have used gold nanoparticles as their sensing platform due to easy synthesis and functionalization of
them. Here, we demonstrate that the aggregation of silver nanoparticles induced by poly-L-lysine molecules can generate
much higher HRS than that from gold nanoparticle aggregates when using a Ti:Sapphire femtosecond laser. In spite of
several drawbacks of silver nanoparticle system regarding the nonlinear response to target concentration and the
difficulty of surface functionalization compared to the gold nanoparticle system, much higher HRS from silver
nanoparticles can be definitely useful for the sensitive detection of very small amount of target molecules.
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