We present a multiscale imaging system in which a conventional microscope stand is inserted in the sample arm of a Spectral-Domain Optical Coherence Microscopy (SD-OCM) system. The instrument was designed to facilitate localisation and maturity assessment of oocytes and early-stage embryos to be used for in vitro fertilisation (IVF), by a versatile and easy switch of microscope objectives. The dispersion variation due to change of the microscope objectives is dealt with by employing the Complex Master Slave procedure that enables correct operation without the use of matching glass in the reference arm for dispersion compensation.
The most successful methods for speckle reduction in Optical Coherence Tomography (OCT) are based on deformation of the wavefront used in scanning. Here, a simple method is presented where the wavefront is distorted by lateral translation of the lens between the 2D galvo-scanner and sample. The report demonstrates that the method can be implemented with a small piezoelectric transducer. Up to 33% improvement in Speckle Contrast Ratio (SCR) of B-scan OCT images is demonstrated.
We present a optical coherence tomography system for minimally-disturbance imaging of porcine embryos, placed inside an incubator which ensures adequate environmental parameters including temperature, humidity, and gas ratios.
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