This study contains analysis of existing approaches for detecting photosensitizer-free laser-induced singlet oxygen, including their ability to quantitatively measure the relationship between a dose of laser radiation and the amount of singlet oxygen produced.
An approach to visualization of the vascular bed with the possibility of assessing changes in blood filling and identifying diagnostically significant periodic changes in the signal by analyzing speckle images is proposed. The effect of singlet oxygen by direct excitation of an oxygen molecule by 1267 nm laser radiation on changes in the vascular bed parameters was studied using this approach.
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