Dr. Martin R. Harris Profile

Dr. Martin R. Harris

at OptiScan Pty Ltd

SPIE Involvement:

Author

Proceedings Article | 14 February 2007 Paper

Fluorescence confocal endomicroscopy in biological imaging

Peter Delaney, Steven Thomas, John Allen, Wendy McLaren, Elise Murr, Martin Harris

Proceedings Volume 6432, 64320G (2007) https://doi.org/10.1117/12.702254

KEYWORDS: Confocal microscopy, Tissues, Animal model studies, Endomicroscopy, In vivo imaging, Image resolution, Laparoscopy, Liver, Imaging systems, Luminescence

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In vivo fluorescence microscopic imaging of biological systems in human disease states and animal models is possible with high optical resolution and mega pixel point-scanning performance using optimised off-the-shelf turn-key devices. There are however various trade-offs between tissue access and instrument performance when miniaturising in vivo microscopy systems. A miniature confocal scanning technology that was developed for clinical human endoscopy has been configured into a portable device for direct hand-held interrogation of living tissue in whole animal models (Optiscan FIVE-1 system). Scanning probes of 6.3mm diameter with a distal tip diameter of 5.0mm were constructed either in a 150mm length for accessible tissue, or a 300mm probe for laparoscopic interrogation of internal tissues in larger animal models. Both devices collect fluorescence confocal images (excitation 488 nm; emission >505 or >550 nm) comprised of 1024 x 1204 sampling points/image frame, with lateral resolution 0.7um; axial resolution 7um; FOV 475 x 475um. The operator can dynamically control imaging depth from the tissue surface to approx 250um in 4um steps via an internally integrated zaxis actuator. Further miniaturisation is achieved using an imaging contact probe based on scanning the proximal end of a high-density optical fibre bundle (~30,000 fibres) of <1mm diameter to transfer the confocal imaging plane to tissue in intact small animal organs, albeit at lower resolution (30,000 sampling points/image). In rodent models, imaging was performed using various fluorescent staining protocols including fluorescently labelled receptor ligands, labelled antibodies, FITC-dextrans, vital dyes and labelled cells administered topically or intravenously. Abdominal organs of large animals were accessed laparoscopically and contrasted using i.v. fluorescein-sodium. Articular cartilage of sheep and pigs was fluorescently stained with calcein-AM or fluorescein. Surface and sub-surface cellular and sub-cellular details could be readily visualised in vivo at high resolution. In rodent disease models, in vivo endomicroscopy with appropriate fluorescent agents allowed examination of thrombosis formation, tumour microvasculature and liver metastases, diagnosis and staging of ulcerative colitis, liver necrosis and glomerulonephritis. Miniaturised confocal endomicroscopy allows rapid in vivo molecular and subsurface microscopy of normal and pathologic tissue at high resolution in small and large whole animal models. Fluorescein endomicroscopy has recently been introduced into the medical device market as a clinical imaging tool in GI endoscopy and is undergoing clinical evaluation in laparoscopic surgery. This medical usage is encouraging in-situ endomicroscopy as an important pre-clinical research tool to observe microscopic and molecular system biologic events in vivo in animal models for various human diseases.

SPIE Journal Paper | 1 January 2007 Open Access

Laser confocal endomicroscopy as a novel technique for fluorescence diagnostic imaging of the oral cavity

Patricia Thong, Malini Olivo, Kiang-Wei Kho, Kent Mancer, Wei Zheng, Martin Harris, Khee-Chee Soo

JBO, Vol. 12, Issue 01, 014007, (January 2007) https://doi.org/10.1117/12.10.1117/1.2710193

KEYWORDS: Luminescence, Tongue, Tissues, Confocal microscopy, Natural surfaces, Endomicroscopy, Animal model studies, Diagnostics, Sodium, Cancer

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Proceedings Article | 25 April 2005 Paper

Confocal fluorescence endomicroscopic imaging of the tongue

Wei Zheng, Malini Olivo, K. W. Kho, Patricia Thong, Martin Harris, Khee Chee Soo

Proceedings Volume 5686, (2005) https://doi.org/10.1117/12.589005

KEYWORDS: Confocal microscopy, Tissues, Luminescence, Tongue, In vivo imaging, Natural surfaces, Cancer, Endomicroscopy, Imaging systems, Endoscopes

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Conference Committee Involvement (4)

Endoscopic Microscopy V

25 January 2010 | San Francisco, California, United States

Endoscopic Microscopy III

20 January 2008 | San Jose, California, United States

Endoscopic Microscopy II

21 January 2007 | San Jose, California, United States

Endoscopic Microscopy

22 January 2006 | San Jose, California, United States

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