Necrotizing soft-tissue infections (NSTIs) are aggressive and deadly. A major obstacle to prompt NSTI management is the lack of a definitive diagnostic test, causing treatment delays and increasing mortality. NSTI-affected tissues undergo prominent blood vessel thrombosis invisible to the naked eye that can be rapidly visualized using perfusion-based fluorescence imaging. To validate fluorescence imaging for rapid NSTI detection, we are developing a preclinical mouse model of NSTI using light-producing strains of causative bacteria that have biological equivalence to wild type. Co-registration of our wide-field fluorescence imagery with spectrally distinct, bacterial bioluminescence provides validation of infection presence and spatial extent.
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