Photodynamic therapy has emerged as a possible supplement to the existing protocols for endodontic
disinfection. Microbes are known to gain significant ecological advantage when they survive as
coaggregates and biofilms in an infected tissue. Such microbial coaggregates and biofilms have been
confirmed to play a key role in the pathogenicity of many infections. So far, not many studies have
correlated the efficacy of antimicrobial photodynamic inactivation (APDI) to the different modes of
bacterial growth. This study aims to evaluate the APDI of 3 strains of Enterococcus faecalis in planktonic
phase, in a co-aggregated suspension and in a 4-day old biofilm. The results showed that the biofilm mode
of growth offered the greatest resistance to APDI and the inclusion of an efflux pump inhibitor significantly
increased the APDI of biofilm bacteria. From this study, we conclude that APDI of bacteria in biofilms is
the most challenging and that the use of bacterial efflux pump inhibitors enhances its photodynamic antibiofilm
efficacy.
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