Metabolic control of immune cell function has emerged as a key regulatory mechanism in immunity. Current studies rely mainly on in vitro and ex vivo approaches to characterize the intracellular metabolism of immune cells. Here, we show that fluorescence lifetime imaging microscopy of metabolic coenzymes, NAD(P)H and FAD, captures dynamic changes in the metabolic activity of macrophages during diverse inflammatory responses in their native interstitial microenvironments. This imaging-based approach also provides single-cell resolution, in contrast to bulk measurements provided by traditional biochemical assays, making it a potentially valuable tool in the field of immunometabolism.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.