Visible/near infrared spectroscopy on-line determination had been widely used in agricultural products and food samples non-destructive internal quality determination. This research proposed to design real-time determination software in order to estimate soluble solids content (SSC) of fruit on line. Functions of the software included real-time spectroscopy pre-processing, real-time spectroscopy viewing, model building, SSC estimating, etc. In addition, Fenghua juicy peaches were used to validate the practicability and the real-time capability. And SSCs of peach samples were predicted by the software on line. The research provided some help to the real-time non-destructive internal quality determination of the fruit. As the important part of the real-time determination, the determination method and technology were fully accordance with the need at real-time and model's precision.
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