We have examined the influence of electrical bias on immobilization of bacteria on the surface of GaAs/AlGaAs heterostructures, functionalized with an alkanethiol based architecture. A mixture of biotinylated polyethylene glycol (PEG) thiol and hexadecanethiol was applied to attach neutravidin and antibodies targeting specific immobilization of Legionella pneumophila. An electrochemical setup was designed to bias biofunctionalized samples with the potential measured versus silver/silver chloride reference electrode in a three electrode configuration system. The immobilization efficiency has been examined with fluorescence microscopy after tagging captured bacteria with fluorescein labeled antibodies. We demonstrate more than 2 times enhanced capture of Legionella pneumophila, suggesting the potential of electrically biased biochips to deliver enhanced sensitivity in detecting these bacteria.
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