Ms. Nan Cheng Profile

Nan Cheng

at Tianjin Univ

SPIE Involvement:

Author

Publications (2)

SPIE Journal Paper | 22 April 2022 Open Access

High-sensitivity dynamic diffuse fluorescence tomography system for fluorescence pharmacokinetics

Limin Zhang, Nan Cheng, Han Liu, Yingxue Pan, Yanqi Zhang, Feng Gao

JBO, Vol. 27, Issue 04, 046002, (April 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.4.046002

KEYWORDS: Luminescence, Liver, Imaging systems, Fluorescence tomography, In vivo imaging, Dynamical systems, Modulation, Tumors, Signal detection, Optical fibers

Read Abstract +

Significance: Dynamic diffuse fluorescence tomography (DFT) can recover the static distribution of fluorophores and track dynamic temporal events related to physiological and disease progression. Dynamic imaging indocyanine green (ICG) approved by the food and drug administration is still under-exploited because of its characteristics of low quantum yield and relatively rapid tissue metabolism. Aim: In order to acquire the ICG tomographic image sequences for pharmacokinetic analysis, a dynamic DFT system was proposed. Approach: A fiber-based dynamic DFT system adopts square-wave modulation lock-in photon-counting scheme and series-parallel measurement mode, which possesses high sensitivity, large dynamic range, high anti-ambient light ability in common knowledge, as well as good cost performance. In order to investigate the effectiveness of the proposed system, the measurement stability and the anti-crosstalk—a crucial factor affecting the system parallelization—were assessed firstly, then a series of static phantoms, dynamic phantoms and in vivo mice experiments were conducted to verify the imaging capability. Results: The system has the limited dynamic range of 100 dB, the fluctuation of photon counting within 3%, and channel-to-channel crosstalk ratio better than 1.35. Under the condition of a sufficient signal-to-noise ratio, a complete measurement time for one frame image was 10.08 s. The experimental results of static phantoms with a single target and three targets showed that this system can accurately obtain the positions, sizes, and shapes of the targets and the reconstructed images exhibited a high quantitativeness. Further, the self-designed dynamic phantom experiments demonstrated the capability of the system to capture fast changing fluorescence signals. Finally, the in vivo experiments validated the practical capability of the system to effectively track the ICG metabolism in living mice. Conclusions: These results demonstrate that our proposed system can be utilized for assessing ICG pharmacokinetics, which may provide a valuable tool for tumor detection, drug assessment, and liver function evaluation.

DOWNLOAD PAPER

Proceedings Article | 10 October 2020 Poster + Paper

Assessing pharmacokinetics of indocyanine green in liver injury mice using dynamic diffuse fluorescence tomography system

Ke Shi, Limin Zhang, Zhichao Zhao, Bin Cao, Nan Chen, Jiao Li, Zhongxing Zhou, Feng Gao

Proceedings Volume 11553, 115532B (2020) https://doi.org/10.1117/12.2574153

KEYWORDS: Liver, Fluorescence tomography, Injuries, Indocyanine green, Mode conditioning cables, Luminescence, Tissues, Biological research, Absorption, Bioalcohols

Read Abstract +

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years