The study highlights the effect of different modes of in vivo laser irradiation of mice using a PFL8LA laser with λ = 1560 nm, pulse duration of 1,4•10-12 s, peak power of 3,72•103 W and average output power of 20•10-3 W on the lipid peroxidation parameters: conjugated dienes, ketodienes and conjugated trienes, malondialdehyde, Schiff bases and the activity of antioxidant enzymes - catalase, glutathione -S-transferase and superoxide dismutase in erythrocytes and plasma of mice. Two groups of mice received a total dose of 3.8 J/cm2 per group, but the 1st group was irradiated only once, while the 2nd – four times. Significant differences in the parameters of the 1st and 2nd groups indicate different effects of the irradiation modes on redox-dependent processes in red blood cells of mice.
The present work discusses effect of infrared (IR) femtosecond laser irradiation on neoplasm of white
mice with experimental cervical cancer- 5 (CC-5 on the 20th and 30th days after tumor transplantation). Tumor
tissue was irradiated by femtosecond erbium doped fiber laser: the wavelength is 1.55 μm, average and peak
powers are1,25 mW and 6kW, respectively, irradiation trials n=10. The average energy density (energy dose)
on a tissue for two groups of animals was 0,24 J/cm2 and 0,36 J/cm2 for a single trial. Irradiation was
followed by biochemical determination of LPO AOS parameters (“Lipid peroxidation-antioxidants” system):
malondialdehyde (MDA), activity of superoxide dismutase (SOD), catalase and glutathione-reductase (GR),
glutathione-S-transferase (GST). A subsequent morphological study of tumor tissue was performed.
Mathematical analysis of data demonstrates a weak dependence of the studied parameters on energy
dose. The latter implies the trigger effect of IR femtosecond laser irradiation on redox-dependent processes in
neoplasm at experimental cervical cancer.
In the present work the effects of high-power femtosecond laser irradiation on a functional condition of red blood cells
and neutrophils in vitro have been investigated. The data on parameters of the lipid peroxidation - antioxidants system,
hemoglobin level and rigidity of red blood cell membranes testify destabilization of the membranes under the influence
of the given laser. The study of phagocytic activity, anaerobic and aerobic metabolism of neutrophils, and rigidity of
their membranes allows to suppose the dose-dependent effect to be stimulating.
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