We present a shot noise limited, three-color SRS implementation to address two molecular vibrations simultaneously. The system allows fast, high quality stimulated Raman histology as well as background-free SRS imaging. It is ready to be tested in hospitals for its viability and image quality in comparison to classical rapid histology.
It is based on a mode-locked fs-laser from which 2 narrow-band Stokes laser beams are extracted and subsequently modulated at 13 and 20MHz. The center part of the fs-laser is frequency doubled to pump a picosecond optical parametric oscillator, which can be tuned from 500 to 5000 cm−1.
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