Mr. Samuel Border Profile

Samuel Border

at Univ of Florida

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Publications (4)

Proceedings Article | 3 April 2024 Presentation + Paper

CODEX and H&E imaging: cell type mapping, analysis, and visualization pipeline

Julio Maragall, Nicholas Lucarelli, Ahmed Naglah, Samuel Border, Seth Winfree, Zoltan Laszik, Michael Eadon, Tarek El-Achkar, Sanjay Jain, Pinaki Sarder

Proceedings Volume 12933, 129330W (2024) https://doi.org/10.1117/12.3008471

KEYWORDS: Image segmentation, Tissues, Feature extraction, Kidney, Visualization, Biological imaging, Image registration, Analytical research, Proteins, Medicine

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Emerging spatially resolved molecular imaging techniques, such as co-detection by indexing (CODEX), have enabled researchers to uncover distinct cellular structures in histological kidney sections. Spatial proteomics can provide users with the intensity level of proteins synthesized in the tissue in the same histology tissue section. However, the mapping of cell type proportions and molecular signatures can be challenging which might have contributed to the limited use of these technologies in clinical practice. Developing a computational model that handles such highdimensional whole-slide imaging (WSI) data from CODEX requires applying advanced machine learning techniques to address common challenges such as interpretability, efficiency, and usability. In this study, we propose a computational pipeline for CODEX mapping on biopsy images that features an automated registration module that utilizes nuclei segmentation in both modalities. Our pipeline provides an explainable prediction and mapping of cell type clusters on histology and analyzes the heterogeneity of molecular features in the predicted clusters. For mapping, we used an unsupervised clustering analysis of uniform manifold approximation and projection (UMAP)- reduced features to enable visualizing the predicted clusters onto the histological tissue image. To test our proposed pipeline, we used a high-dimensional CODEX panel that comprises 44 markers and visualized the intensities and the predicted clusters on whole slide images (WSI) in a set of renal histology samples collected atIndiana University. Our results delineated 14 distinct cell clusters which demonstrated high fidelity between labeled objects and specific markers. Notably, 88% of cells in the “podocytes” dominant UMAP cluster were found to have a high level of podocalyxin, although it is adjacent to two other clusters dominated by renal vasculature cells. Out of 626 features examined, 44 were central to the “podocyte” cluster, accounting for approximately 50% of its variance (p < 0.05). This study can improve the understanding of the cell type proportions and kidney functions of tissue structures, which can contribute to the human biomolecular kidney atlas; a step towards substantial advancements in the field of kidney cell biology research.

Proceedings Article | 3 April 2024 Presentation + Paper

ComPRePS: an automated cloud-based image analysis tool to democratize AI in digital pathology

Sayat Mimar, Anindya Paul, Nicholas Lucarelli, Samuel Border, Ahmed Naglah, Laura Barisoni, Jeffrey Hodgin, Avi Rosenberg, William Clapp, Pinaki Sarder

Proceedings Volume 12933, 129330Z (2024) https://doi.org/10.1117/12.3008469

KEYWORDS: Artificial intelligence, Image segmentation, Pathology, Education and training, Image analysis, Data modeling, Visualization, Feature extraction, Medicine, Process modeling

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Proceedings Article | 13 March 2024 Presentation + Paper

Utilizing DUET imaging for quantification of collagen and elastin as biomarkers for studying disease

Willy Ju, Samuel Border, Anupam Mitra, Eniola Okon, Hyunjae Jeong, Richard Levenson, Avi Rosenberg, Kuang-Yu Jen, Pinaki Sarder, Farzad Fereidouni

Proceedings Volume 12862, 1286206 (2024) https://doi.org/10.1117/12.3009357

KEYWORDS: Collagen, Arteries, Fluorescence, Vascular diseases, Kidney, Tumors, Tissues, Diseases and disorders, Computer aided detection, Pathology

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Proceedings Article | 7 April 2023 Presentation + Paper

Improving quantification of renal fibrosis using deep-DUET

Samuel Border, Avi Rosenberg, Jarcy Zee, Richard Levenson, Kuang-Yu Jen, Pinaki Sarder, Farzad Fereidouni

Proceedings Volume 12471, 124710G (2023) https://doi.org/10.1117/12.2654651

KEYWORDS: Collagen, Fluorescence, Image segmentation, Data modeling, Tissues, Pathology, Diseases and disorders, Biopsy, Reproducibility

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