Dr. Setumo Lebogang S. Thobakgale Profile

Dr. Setumo Lebogang S. Thobakgale

Doctoral Candidate at CSIR National Laser Ctr

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Author

Publications (12)

Proceedings Article | 27 March 2024 Presentation + Paper

Detection of viral pathogens using optical photonic techniques with the aid of selenium nanoparticles

Z. K. Mcotshana, L. Thwala, S. Ombinda-Lemboumba, R. Ramokolo, S. Thobakgale, M. Lugongolo, E. Van Steen, P. Mthunzi-Kufa

Proceedings Volume 12850, 1285008 (2024) https://doi.org/10.1117/12.3002381

KEYWORDS: Nanoparticles, Selenium, Antibodies, Localized surface plasmon resonances, Spectroscopy, Raman spectroscopy, Optical transmission, Glasses, Biosensors, YAG lasers

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Viral infections such as HIV and SARS-CoV-2 have significantly increased morbidity in humans and resulted in a significant number of fatalities globally, hence early detection is crucial, particularly at a point-of-care (POC) setting to prevent the spread of these diseases. Localized surface plasmon resonance (LSPR) and green light-based Transmission spectroscopy techniques were used in this study to assess real-time molecular interactions between virus-spiked and nonspiked samples. The current study focuses on integrating selenium nanoparticles (SeNPs) with different optical photonic techniques for enhanced detection of HIV. Selenium nanoparticles were synthesized and functionalized with antibodies specific to HIV. Before and after bioconjugation with viral secondary antibodies, the SeNPs were characterized using Ultraviolet–visible (UV-Vis) spectroscopy, Dynamic light scattering (DLS), High-resolution transmission electron microscopy (HRTEM), and Raman spectroscopy, to elucidate their properties and confirm the presence of functional groups. After that, the NPs were integrated with plasmonic systems and used for the enhanced detection of HIV in comparison to traditional LSPR and Transmission spectroscopy. Colloidal selenium nanoparticles were successfully synthesized, using ND: YAG laser. The orange-colored, spherically shaped nanoparticles were evenly distributed and easily resuspended. Anti-HIV antibodies conjugated to SeNPs were added after HIV-specific antibodies were successfully immobilized on a glass slide substrate to react with HIV pseudovirus. The pseudovirus was effectively identified by the use of Transmission Spectroscopy and LSPR techniques. The two optical techniques for HIV detection were more sensitive after integrating selenium nanoparticles, as compared to the conventional Transmission spectroscopy and LSPR methods. This improved and highly sensitive approach may be utilized to identify viral infections early, thus combating the spread of infectious diseases.

Proceedings Article | 5 March 2021 Presentation + Paper

Label-free detection of mutations in the HIV genome using a surface plasmon resonance biosensor

M. Lugongolo, S. Manoto, C. Maphanga, S. Ombinda-Lemboumba, S. Thobakgale, P. Mthunzi-Kufa

Proceedings Volume 11661, 116610M (2021) https://doi.org/10.1117/12.2578317

KEYWORDS: Surface plasmons, Biosensors, Sensors, Resistance, Refractive index, Polymers, Optical testing, Molecular interactions

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Proceedings Article | 2 March 2020 Presentation + Paper

Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy

Lebogang Thobakgale, Sello Manoto, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 11269, 1126905 (2020) https://doi.org/10.1117/12.2546479

KEYWORDS: Raman spectroscopy, Surface enhanced Raman spectroscopy, Thin films, Biosensors, Gold, Calibration

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Proceedings Article | 21 February 2020 Paper

Immobilization of HIV GP41 antibodies on glass substrates for HIV biosensing

Sello Manoto, Ahmed El-Hussein, Rudzani Malabi, Lebogang Thobakgale, Saturnin Ombinda-Lemboumba, Yasser Attia, Mohammed Kasem, Patience Mthunzi-Kufa

Proceedings Volume 11258, 112580O (2020) https://doi.org/10.1117/12.2546497

KEYWORDS: Glasses, Raman spectroscopy, Biosensing, Nanoparticles, Atomic force microscopy, Biosensors, Water, Physics, Chemistry, Proteins

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Proceedings Article | 20 February 2020 Paper

Raman spectroscopy and gold thin film for biosensing and detection

Saturnin Ombinda-Lemboumba, Sello Manoto, Charles Maphanga, Rudzani Malabi, Lebogang Thobakgale, Masixole Lugongolo, Patience Mthunzi-Kufa

Proceedings Volume 11251, 112512P (2020) https://doi.org/10.1117/12.2546617

KEYWORDS: Raman spectroscopy, Gold, Nanoparticles, Glasses, Raman scattering, Sensors, Surface enhanced Raman spectroscopy, Molecules, Thin films, Biosensing

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Proceedings Article | 13 February 2020 Presentation + Paper

Surface enhanced raman spectroscopy on polymer-graphene oxide scaffolds for drug screening applications

Lebogang Thobakgale, Sello Manoto, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 11246, 112460X (2020) https://doi.org/10.1117/12.2546455

KEYWORDS: Graphene, Raman spectroscopy, Oxides, Glasses, Surface enhanced Raman spectroscopy, Polymers, Nanomaterials, Coating, Molecules, Molecular self-assembly

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Proceedings Article | 22 February 2019 Paper

Optimizing and characterizing a home-built Raman spectroscopy optical system using polystyrene microspheres

Lebogang Thobakgale, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa, Rudzani Malabi

Proceedings Volume 10884, 1088416 (2019) https://doi.org/10.1117/12.2509944

KEYWORDS: Raman spectroscopy, Light scattering, Molecules, Data processing, Raman scattering, Polymers, Scattering, CCD cameras, Statistical analysis, Polarizability

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Proceedings Article | 20 February 2019 Presentation + Paper

Investigation and calibration of non-essential amino acids using a custom built Raman spectroscopy system

Lebogang Thobakgale, Saturnin Ombinda Lemboumba, Patience Mthunzi-Kufa

Proceedings Volume 10885, 108850N (2019) https://doi.org/10.1117/12.2509839

KEYWORDS: Raman spectroscopy, Proteins, Calibration, Luminescence, Signal detection, Nanomaterials, Remote sensing, Standards development, Raman scattering, Point-of-care devices

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Proceedings Article | 13 February 2018 Presentation + Paper

Femtosecond laser assisted photo-transfection and differentiation of mouse embryonic stem cells

Lebogang Thobakgale, Sello Manoto, Satuurnin Ombinda Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10492, 1049205 (2018) https://doi.org/10.1117/12.2290206

KEYWORDS: Stem cells, Luminescence, Acquisition tracking and pointing, Femtosecond phenomena, Green fluorescent protein, Microscopes, Pulsed laser operation, Statistical analysis, Organisms, Genetics

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Proceedings Article | 22 February 2017 Paper

Investigation of HIV-1 infected and uninfected cells using the optical trapping technique

S. Ombinda-Lemboumba, R. Malabi, M. Y. Lugongolo, S. Thobakgale, S. Manoto, P. Mthunzi-Kufa

Proceedings Volume 10075, 100750M (2017) https://doi.org/10.1117/12.2252294

KEYWORDS: Optical tweezers, Objectives, Spectroscopy, Transmittance, Optical spectroscopy, Chemical analysis, Laser spectroscopy, Absorption, Biological research, Molecular spectroscopy

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Proceedings Article | 16 February 2017 Paper

Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: differentiation studies

Lebogang Thobakgale, Sello Lebohang Manoto, Satuurnin Ombinda Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10068, 100681U (2017) https://doi.org/10.1117/12.2252255

KEYWORDS: Stem cells, Tissue engineering, Molecules, Biological research, Luminescence, Acquisition tracking and pointing, Femtosecond phenomena, Laser induced fluorescence, Genetics, Tissues, Absorbance, Receptors

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Proceedings Article | 15 February 2017 Presentation + Paper

Phototransfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses

Lebogang Thobakgale, Sello Lebohang Manoto, Saturnin Ombinda Lemboumba, Malik Maaza, Patience Mthunzi-Kufa

Proceedings Volume 10062, 1006206 (2017) https://doi.org/10.1117/12.2252188

KEYWORDS: Femtosecond phenomena, Stem cells, Genetics, Tissues, Molecules, Tissue engineering, Laser irradiation, Laser ablation, Optical properties, Luminescence, Acquisition tracking and pointing, Absorbance, Pulsed laser operation, Proteins, Objectives, Chemical analysis

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Showing 5 of 12 publications

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