Dr. Stefan Schinkinger Profile

Dr. Stefan Schinkinger

at Univ Leipzig

SPIE Involvement:

Author

Publications (2)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 25 February 2006 Paper

Feeling with light for cancer

Mireille Martin, Karla Mueller, Falk Wottawah, Stefan Schinkinger, Bryan Lincoln, Maren Romeyke, Josef Käs

Proceedings Volume 6080, 60800P (2006) https://doi.org/10.1117/12.637899

KEYWORDS: Cytoskeletons, Cancer, Tissue optics, Microfluidics, Biomedical optics, Tumors, Optical tweezers, Chemical elements, Refractive index, Protactinium

Read Abstract +

Even minute alterations in a cell's intracellular scaffolds, i.e. the cytoskeleton, which organize a cell, result in significant changes in a cell's elastic strength since the cytoskeletal mechanics nonlinearly amplify these alterations. Light has been used to observe cells since Leeuwenhoek's times and novel techniques in optical microscopy are frequently developed in biological physics. In contrast, with the optical stretcher we use the forces caused by light described by Maxwell's surface tensor to feel cells. Thus, the stretcher exemplifies the other type of biophotonic devices that do not image but manipulate cells. The optical stretcher uses optical surface forces to stretch cells between two opposing laser beams, while optical gradient forces, which are used in optical tweezers, play a minor role and only contribute to a stable trapping configuration. The combination of the optical stretcher's sensitivity and high throughput capacity make a cell's "optical stretchiness" an extremely precise parameter to distinguish different cell types. This avoids the use of expensive, often unspecific molecular cell markers. This technique applies particularly well to cells with dissimilar degrees of differentiation, as a cell's maturation correlates with an increase in cytoskeletal strength. Because malignant cells gradually dedifferentiate during the progression of cancer, the optical stretcher should allow, the direct staging from early dysplasia to metastasis of a tumor sample obtained by MRI-guided fine needle aspirations or cytobrushes. With two prototypes of a microfluidic optical stretcher at our hands, we prepare preclinical trials to study its potential in resolving breast tumors' progression towards metastasis. Since the optical stretcher represents a basic technology for cell recognition and sorting, an abundance of further biomedical applications can be envisioned.

Proceedings Article | 18 October 2004 Paper

Feeling for cells with light

Stefan Schinkinger, Falk Wottawah, Kort Travis, Bryan Lincoln, Jochen Guck

Proceedings Volume 5514, (2004) https://doi.org/10.1117/12.556795

KEYWORDS: Geometrical optics, Protactinium, Cytoskeletons, Dielectrics, Refractive index, Interfaces, Spherical lenses, Microfluidics, Statistical analysis, Cancer

Read Abstract +

In an optical stretcher, infrared laser light is used to exert surface stress on biological cells, causing an elongation of the trapped cell body along the laser beam axis. These optically induced deformations characterize individual cells and cell lines. When integrated within a microfluidic chamber with high throughput, this enables diagnosis of diseases, on a cellular level, that are associated with cytoskeletal processes. Additionally, it allows sorting of cells with high accuracy in a non-contact manner. To determine the surface stress on the cell, ray optics calculations as well as the system transfer operator (T-matrix) approach with an appropriate incident field are used. The latter approach allows a more accurate modeling of the cell in the optical stretcher and reveals a more detailed stress profile acting on the cell surface. Analyzing the deformation behavior of normal and malignantly transformed fibroblasts, significant differences in axial elongation even for sample sizes as low as 30 cells are already measurable on a time scale of 0.1s. Here, malignant transformation of cells is discussed as an example of how any process that affects the cell's optical or mechanical properties allows classification with the optical stretcher.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years