Prof. Yong Zhang Profile

Prof. Yong Zhang

at Univ of North Carolina at Charlotte

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Proceedings Article | 21 February 2020 Presentation + Paper

Light-assisted drying (LAD) for anhydrous preservation of biologics: using Raman spectroscopy to assess the uniformity of drying in processed samples

Riley McKeough, Daniel Furr, Fan Zhang, Yong Zhang, Susan Trammell

Proceedings Volume 11230, 112300K (2020) https://doi.org/10.1117/12.2546027

KEYWORDS: Proteins, Raman spectroscopy, Solids, Glasses, Humidity, Cameras, Matrices, Near infrared spectroscopy, Laser processing, Photonics

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Protein-based therapeutics have been developed to treat a range of conditions and assays use immobilized capture proteins for the detection of diseases. A challenge in the development of protein-based products is maintaining the protein in the folded state during processing and storage. The most common method of stabilizing proteins for storage is lyophilization. However, the freeze-drying process remains expensive and many proteins that are lyophilized must be refrigerated or frozen to maintain functionality. Cold storage strategies can be challenging for the transportation of protein-based products and can be difficult or impossible in low resource settings. Recent research has demonstrated that anhydrous, or dry state, preservation in a trehalose amorphous solid matrix offers an alternative to freeze drying for the preservation of biologics. We have previously described a new processing technique, light assisted drying (LAD), to create trehalose preservation matrices. LAD uses illumination by near-infrared laser light to selectively heat water and speeds dehydration of small volume (40 μL) samples. Low end moisture contents (EMC’s) are necessary for storage at supra-zero temperatures and this low water content must be uniform to insure successful long-term storage of embedded biologics. Our previous work has demonstrated the ability of LAD to reach EMCs necessary for storage at elevated temperatures. In this work, Raman spectroscopy is used to assess the trehalose distribution and water content across LAD processed samples. Results indicate that the water content of LAD process samples is uniform. LAD is a promising technique for processing biologics in preparation for anhydrous storage.

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