The stems of Actinidia Qinmei were taken as explants and induced callus formation after having gained the aseptic
seedling in primary culture successfully, and then the calli were placed on different combinations regeneration medium.
The results showed that the induction medium added 1 mg/L 6-BA and 0.1 mg/L NAA was beneficial to the callus
induction in dark culture condition. The suitable regeneration medium was MS containing 2.0 mg/L 6BA, 0.1 mg/L
NAA and 3% (W/V) sucrose, it could improve obviously the frequency of regenerated shoots. This method separated the
callus induction from shoot regeneration and obtained more callus to optimize the regenerated medium, and also was
advantageous to Kiwifruit genetic transformation.
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