Multifocal structured illumination optoacoustic microscopy

Zhenyue Chen; Xosé Luis Deán-Ben; Ali Özbek; Johannes Rebling; Daniel Razansky

doi:10.1117/12.2508834

27 February 2019 Multifocal structured illumination optoacoustic microscopy

Zhenyue Chen, Xosé Luis Deán-Ben, Ali Özbek, Johannes Rebling, Daniel Razansky

Proceedings Volume 10878, Photons Plus Ultrasound: Imaging and Sensing 2019; 1087816 (2019) https://doi.org/10.1117/12.2508834
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Optoacoustic imaging is a highly scalable and versatile method that can be used for optical resolution (OR) microscopy applications at superficial depth yet can be adapted for tomographic imaging with ultrasonic resolution at centimeter penetration scales. However, imaging speed of the commonly employed scanning-based microscopy methods is slow as far as concerned with acquisition of volumetric data. Herein, we propose a new approach using multifocal structured illumination in conjunction with a spherical matrix ultrasonic array detection to achieve fast volumetric optoacoustic imaging in both optical and acoustic resolution modes. In our approach, the laser beam is raster scanned by an acousto-optic deflector running at hundred hertz scanning rate with the beam then split into hundreds of mini-beams by a beamsplitting grating, which are subsequently focused by a condensing lens to generate multifocal structured illumination. Phantom experimental results show that 10 x 10 x 5 cm³ volumetric imaging can be accomplished with spatial resolution around 29 μm. We believe by further speeding up the data acquisition in the further, the system will be operated in full power, making it possible to study functional, kinetic and metabolic processes across multiple penetration scales.

Conference Presentation

Citation Download Citation

Zhenyue Chen, Xosé Luis Deán-Ben, Ali Özbek, Johannes Rebling, and Daniel Razansky "Multifocal structured illumination optoacoustic microscopy", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 1087816 (27 February 2019); https://doi.org/10.1117/12.2508834

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available