Multimodal microscopy toolkit for visualizing multicomponent topical drug formulations in humans

Alexander Fast; Sinyoung Jeong; Maiko Hermsmeier; Akira Yamamoto; Xin Chen; Kin F. Chan; Conor L. Evans

doi:10.1117/12.2510157

5 March 2019 Multimodal microscopy toolkit for visualizing multicomponent topical drug formulations in humans

Alexander Fast, Sinyoung Jeong, Maiko Hermsmeier, Akira Yamamoto, Xin Chen, Kin F. Chan, Conor L. Evans

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108822J (2019) https://doi.org/10.1117/12.2510157
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Our group has recently developed a method for characterizing distribution of a topical drug within skin using two-photon fluorescence lifetime imaging (FLIM) and phasor analysis. Here, we expand on this work by describing a multimodal approach for rapidly visualizing multiple components in tissue using FLIM and coherent Raman imaging (CRI). By employing a non-Euclidian FLIM phasor analysis for a three-component system informed with the vibrational signature of one of the components retrieved with CRI, we were able to semi-quantitatively describe the spatial distribution of drugs in tissue with molecular specificity and cellular resolution.

Citation Download Citation

Alexander Fast, Sinyoung Jeong, Maiko Hermsmeier, Akira Yamamoto, Xin Chen, Kin F. Chan, and Conor L. Evans "Multimodal microscopy toolkit for visualizing multicomponent topical drug formulations in humans", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108822J (5 March 2019); https://doi.org/10.1117/12.2510157

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KEYWORDS

Tissues

Fluorescence lifetime imaging

Visualization

Raman spectroscopy

Skin

Confocal microscopy

Microscopy

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