Multiphoton imaging of autofluorescent advanced glycation end product formation in porcine aorta

Chih-Ju Lin; Jeon Woong Kang; Peter T. C. So; Chen-Yuan Dong

doi:10.1117/12.2583607

25 March 2021 Multiphoton imaging of autofluorescent advanced glycation end product formation in porcine aorta

Chih-Ju Lin, Jeon Woong Kang, Peter T. C. So, Chen-Yuan Dong

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 1164815 (2021) https://doi.org/10.1117/12.2583607
Event: SPIE BiOS, 2021, Online Only

Abstract

Exposure of tissues to sugar lead to the formation of advanced glycation end products (AGEs), contributing to diabetic complications. In human physiology, the vasculature is in direct contact with blood, thus the effect of diabetes is expected to be most severe with the vasculature. In this study, we incubated excised porcine aorta in D-glucose, Dgalactose, and D-fructose solutions. Multiphoton microscopy shows that for Days 4 to 48 incubation, autofluorescence is constant along the aorta sections, suggesting that monosaccharide diffusion is rapid when compared to the rate of fluorescent AGE formation.

Conference Presentation

Citation Download Citation

Chih-Ju Lin, Jeon Woong Kang, Peter T. C. So, and Chen-Yuan Dong "Multiphoton imaging of autofluorescent advanced glycation end product formation in porcine aorta", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 1164815 (25 March 2021); https://doi.org/10.1117/12.2583607

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