Fusing quantitative-phase imaging with airy light-sheet microscopy

N. R. Subedi; P. S. Jung; E. L. Bredeweg; S. Nemati; S. E. Baker; D. N. Christodoulides; A. E. Vasdekis

doi:10.1117/12.2583926

5 March 2021 Fusing quantitative-phase imaging with airy light-sheet microscopy

N. R. Subedi, P. S. Jung, E. L. Bredeweg, S. Nemati, S. E. Baker, D. N. Christodoulides, A. E. Vasdekis

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116530I (2021) https://doi.org/10.1117/12.2583926
Event: SPIE BiOS, 2021, Online Only

Abstract

We report the integration of quantitative-phase imaging (QPI) with light-sheet (LS) fluorescent microscopy on to a standard inverted microscope that retains compatibility with microfluidics. QPI enables label-free imaging and number-density quantification of single cells and their organelles. Conversely, LS yields considerable speed and phototoxicity gains in quantifying the 4D dynamics of gene-encoded fluorescent biomarkers. We will detail the system design that relied on spatial light interferometry for QPI and an accelerating Airy-beam light-sheet for fluorescence, its performance, as well as results of a representative multivariate imaging analysis of single-cell metabolism.

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Citation Download Citation

N. R. Subedi, P. S. Jung, E. L. Bredeweg, S. Nemati, S. E. Baker, D. N. Christodoulides, and A. E. Vasdekis "Fusing quantitative-phase imaging with airy light-sheet microscopy", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116530I (5 March 2021); https://doi.org/10.1117/12.2583926

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