High speed focus-shift for light sheet microscope using continuous rotating planar mirrors

Kenneth Li; Bi-Chang Chen; Po-Yen Lin

doi:10.1117/12.2609086

2 March 2022 High speed focus-shift for light sheet microscope using continuous rotating planar mirrors

Kenneth Li, Bi-Chang Chen, Po-Yen Lin

Proceedings Volume 11971, High-Speed Biomedical Imaging and Spectroscopy VII; 1197105 (2022) https://doi.org/10.1117/12.2609086
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

The continuous rotating planar mirror-based focus-shift system can be used for various microscopes including light-sheet, confocal, fluorescent, and others. The uniform rotation provides a fast, quiet and stable operation without the normal vibration and noise produced by reciprocal motion system. The planar mirrors produce images without the aberrations produced by the Electronically Tunable Lens (ETL) systems. In a light sheet illumination system for 3D applications, high speed change in the focal plane is required such that the imaging portion of the system is synchronized to the scanning light sheet with a stationary sample. A series of 2D images at different times are captured by the digital camera, which are converted into z-axis information and displayed as 3D images.

Conference Presentation

Citation Download Citation

Kenneth Li, Bi-Chang Chen, and Po-Yen Lin "High speed focus-shift for light sheet microscope using continuous rotating planar mirrors", Proc. SPIE 11971, High-Speed Biomedical Imaging and Spectroscopy VII, 1197105 (2 March 2022); https://doi.org/10.1117/12.2609086

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