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29 April 2022 Revealing kinetics of chemical transitions in colorimetric indicators of microorganisms growth based on photometric data from a portable microbiological analyser
Alexander V. Sychev, Roman N. Belenkov, Dmitry N. Ukolov, Artem V. Budaev, Anastasia I. Lavrova, Eugene B. Postnikov
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Proceedings Volume 12194, Computational Biophysics and Nanobiophotonics; 121940Z (2022) https://doi.org/10.1117/12.2625794
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation
Abstract
Here we report the construction, results of testing, and methodology of data processing for a newly developed portable microbiological analyzer (patent application RU2021111818,24.04.2021). This Arduino-based setup allows for high-frequency temporal resolution of data recordings that reduces instant noises and provides detailed dynamical curves. It was tested with the culture of baker’s yeast Saccharomyces cerevisiae in a liquid medium with the usage of resazurin as a redox indicator changing its colour. Moreover, for such fast-growing culture, one should take into account the full chain of chemical transitions from resazurin to resarufin and further to dihydroresorufin. We argue that the respective model of such chemical cascade can be treated with the logletdecomposition of the recorded data into components of two Bertalanffy’s models and demonstrate the ability to define the required kinetic rates.
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Alexander V. Sychev, Roman N. Belenkov, Dmitry N. Ukolov, Artem V. Budaev, Anastasia I. Lavrova, and Eugene B. Postnikov "Revealing kinetics of chemical transitions in colorimetric indicators of microorganisms growth based on photometric data from a portable microbiological analyser", Proc. SPIE 12194, Computational Biophysics and Nanobiophotonics, 121940Z (29 April 2022); https://doi.org/10.1117/12.2625794
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KEYWORDS
Chemical analysis
Biological research
Microorganisms
Yeast
Mathematical modeling
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