High-resolution confocal transmission microscope, Part I: system design

Carol J. Cogswell; John W. O'Byrne

doi:10.1117/12.59581

26 June 1992 High-resolution confocal transmission microscope: Part I - system design

Carol J. Cogswell, John W. O'Byrne

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59581
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

We are investigating using a transmitted light configuration in our experimental confocal microscope, in order to observe highly transparent biological preparations which typically yield little or no signal in reflection. Because refractive index and thickness variations in the specimen often deflect the focused image spot off-axis in a confocal transmission configuration, we are exploring using a CCD array detector in the transmitted beam path instead of a single-pinhole detector. Our method for detecting the brightest pixel values from the CCD diffraction image is shown to give a similar two-point response to that of a confocal fixed-pinhole transmission system. We also discuss the differences between reflection and transmission confocal modes and analyze the relative imaging properties of each.

Citation Download Citation

Carol J. Cogswell and John W. O'Byrne "High-resolution confocal transmission microscope: Part I - system design", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59581

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