In vivo uptake and biodistribution of lipophilic and hydrophilic photosensitizers

Karin Kunzi-Rapp; Nermin Akguen; Herbert Schneckenburger; Angelika C. Rueck; Rudolf W. Steiner

doi:10.1117/12.260754

4 December 1996 In vivo uptake and biodistribution of lipophilic and hydrophilic photosensitizers

Karin Kunzi-Rapp, Nermin Akguen, Herbert Schneckenburger, Angelika C. Rueck, Rudolf W. Steiner

Proceedings Volume 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II; (1996) https://doi.org/10.1117/12.260754
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Uptake and biodistribution of photosensitizers are crucial parameters for evaluating the efficacy of photodynamic therapy (PDT). We used the tumor baring chorioallantoic membrane (CAM) as an in vivo model system to study biodistribution of hydrophilic and lipophile compounds of natural porphyrins and pthalocyanines using confocal laser scanning microscopy (LSM) and time-gated microspectrometry. Simultaneously we observed tumor vessels and tumor cells after intravascular application at different incubation times. Differences in biodistribution of hydrophilic and lipophilic sensitizers could be observed. Hydrophilic compounds were characterized by selective accumulation in tumor cells. In contrast, more lipophilic sensitizers are accumulated in endothelial cells at short incubation times as well as in tumor cells. These facts are in correlation with our studies in tumor cells and endothelial cells in vitro, where hydrophilic sensitizers are characterized by low uptake and low phototoxicity towards endothelial cells. In contrast more lipophilic sensitizers were rapidly taken up and showed high phototoxicity.

Citation Download Citation

Karin Kunzi-Rapp, Nermin Akguen, Herbert Schneckenburger, Angelika C. Rueck, and Rudolf W. Steiner "In vivo uptake and biodistribution of lipophilic and hydrophilic photosensitizers", Proc. SPIE 2924, Photochemotherapy: Photodynamic Therapy and Other Modalities II, (4 December 1996); https://doi.org/10.1117/12.260754

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