Prof. Rudolf W. Steiner Profile

Prof. Rudolf W. Steiner

Professor/Doctor at Univ Ulm

SPIE Involvement:

Author

Publications (69)

Proceedings Article | 24 May 2018 Paper

Multimodal fluorescence imaging navigation for surgical guidance of malignant tumors in photosensitized tissues of neural system and other organs

Maxim Loshchenov, Walter Blondel , Marine Amouroux , Rudolf Steiner , Aleksandr Potapov , Denis Golbin, Oleg Loran, Akobirkhon Babaev , Aleksandr Borodkin, Christian Daul, Dinh-Hoan Trinh, Victor Loschenov

Proceedings Volume 10677, 106773N (2018) https://doi.org/10.1117/12.2311273

KEYWORDS: Luminescence, Cameras, Tumors, Endoscopy, Video, Endoscopes, Surgery, Light sources

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Proceedings Article | 15 July 2015 Paper

Raman and fluorescence microscopy to study the internalization and dissolution of photosensitizer nanoparticles into living cells

Claudia Scalfi-Happ, Rudolf Steiner, Rainer Wittig, Susanna Graefe, Anastasia Ryabova, Victor Loschenov

Proceedings Volume 9537, 953705 (2015) https://doi.org/10.1117/12.2183726

KEYWORDS: Luminescence, Raman spectroscopy, Nanoparticles, Microscopy, Confocal microscopy, Photodynamic therapy, Imaging spectroscopy, Signal detection, Laser scanners, Crystals

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Proceedings Article | 2 March 2015 Paper

Crystalline organic nanoparticles for diagnosis and PDT

R. Steiner, J. Breymayer, Angelika Rueck, V. Loschenov, A. Ryabova

Proceedings Volume 9308, 93080R (2015) https://doi.org/10.1117/12.2080773

KEYWORDS: Luminescence, Nanoparticles, Photodynamic therapy, Skin, Aluminum, Tissues, Crystals, Molecules, Tumors, Tissue optics

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Proceedings Article | 15 February 2008 Paper

SLIM for multispectral FRET imaging

A. Rück, F. Dolp, R. Steiner, C. Steinmetz, B. von Einem, C.A.F. von Arnim

Proceedings Volume 6860, 68601F (2008) https://doi.org/10.1117/12.761660

KEYWORDS: Fluorescence resonance energy transfer, Proteins, Luminescence, Fluorescence lifetime imaging, Multispectral imaging, Alzheimer's disease, Molecules, Sensors, Fluorescence spectroscopy, Resonance energy transfer

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Proceedings Article | 12 July 2007 Paper

Confocal Raman microscopy for investigation of the level of differentiation in living neuroblastoma tumor cells

Claudia Scalfi-Happ, Andrea Jauss, Olaf Hollricher, Simone Fulda, Carmen Hauser, Rudolf Steiner, Angelika Rück

Proceedings Volume 6630, 66300T (2007) https://doi.org/10.1117/12.729455

KEYWORDS: Raman spectroscopy, Confocal microscopy, Microscopy, Tumors, Chemical analysis, Imaging spectroscopy, Microscopes, Data acquisition, Proteins, Nd:YAG lasers

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The investigation of living cells at physiological conditions requires very sensitive, sophisticated, non invasive methods. In this study, Raman spectral imaging is used to identify different biomolecules inside of cells. Raman spectroscopy, a chemically and structurally sensitive measuring technique, is combined with high resolution confocal microscopy. In Raman spectral imaging mode, a complete Raman spectrum is recorded at every confocal image point, giving insight into the chemical composition of each sample compartment. Neuroblastoma is the most common solid extra-cranial tumor in children. One of the unique features of neuroblastoma cells is their ability to differentiate spontaneously, eventually leading to complete remission. Since differentiation agents are currently used in the clinic for neuroblastoma therapy, there is a special need to develop non-invasive and sensitive new methods to monitor neuroblastoma cell differentiation. Neuroblastoma cells at different degrees of differentiation were analysed with the confocal Raman microscope alpha300 R (WITec GmbH, Germany), using a frequency doubled Nd:YAG laser at 532 nm and 10 mW for excitation. Integration time per spectrum was 80-100 ms. A lateral resolution in submicrometer range was achieved by using a 60x water immersion lens with a numerical aperture of 1,0. Raman images of cells were generated from these sets of data by either integrating over specific Raman bands, by basis analysis using reference spectra or by cluster analysis. The automated evaluation of all spectra results in spectral unmixed images providing insight into the chemical composition of the sample. With these procedures, different cell organelles, cytosol, membranes could be distinguished. Since neuroblastoma cells at high degree of differentiation overproduce noradrenaline, an attempt was made to trace the presence of this neurotransmitter as a marker for differentiation. The results of this work may have applications in the monitoring of molecular changes and distribution of biomolecules and in particular of low molecular weight markers as they occur during the differentiation of neuroblastoma cells.

Proceedings Article | 12 February 2007 Paper

SLIM: A sophisticated method for molecular imaging

A. Rück, F. Dolp, R. Steiner, S. Lintner, B. V. Einem, C. V. Arnim

Proceedings Volume 6442, 64420Y (2007) https://doi.org/10.1117/12.714467

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Semiconductor lasers, Fluorescence lifetime imaging, Proteins, Picosecond phenomena, Molecules, Microscopes, Spectrographs, Pulsed laser operation

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Proceedings Article | 12 September 2006 Paper

Optical coherence tomography versus ultrasound for diagnostics in dermatology

Rudolf Steiner, Karin Kunzi-Rapp

Proceedings Volume 6284, 62840E (2006) https://doi.org/10.1117/12.714157

KEYWORDS: Optical coherence tomography, Skin, Ultrasonography, Particles, Scattering, Tissues, Tissue optics, Diagnostics, Point spread functions, Tumors

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Proceedings Article | 8 April 2005 Paper

Photodynamic efficacy and spectroscopic properties of 5-ALA-induced protoporphyrin IX in human glioblastoma cells

Reinhard Sailer, Wolfgang Strauss, Helmut Emmert, Michael Wagner, Rudolf Steiner, Herbert Schneckenburger

Proceedings Volume 5689, (2005) https://doi.org/10.1117/12.590120

KEYWORDS: Luminescence, Breast cancer, Ovarian cancer, Molecules, Brain, Fluorescence lifetime imaging, Photodynamic therapy, Spectroscopy, Fluorescence spectroscopy, Picosecond phenomena

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Proceedings Article | 16 October 2003 Paper

Er:YAG laser for the surgical treatment of carpal tunnel syndrome

Detlef Russ, Thomas Ebinger, Wolfgang Illich, Rudolf Steiner

Proceedings Volume 5142, (2003) https://doi.org/10.1117/12.501157

KEYWORDS: Er:YAG lasers, Nerve, Sapphire, Laser ablation, Endoscopy, Surgery, Laser tissue interaction, Tissues, Laser therapeutics, Collagen

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Proceedings Article | 13 October 2003 Paper

Fluorescence techniques in biomedical diagnostics

Herbert Schneckenburger, Reinhard Sailer, Karl Stock, Wolfgang Strauss, Rudolf Steiner

Proceedings Volume 5068, (2003) https://doi.org/10.1117/12.518752

KEYWORDS: Luminescence, Energy transfer, Fluorescence spectroscopy, Microscopy, Fluorescence lifetime imaging, Molecules, Time resolved spectroscopy, Fluorescence resonance energy transfer, Absorption, Molecular energy transfer

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Proceedings Article | 9 October 2003 Paper

Time-resolved microspectrofluorometry and fluorescence lifetime imaging using ps-pulsed diode lasers in laser scanning microscopes

Angelika Rueck, Frank Dolp, Claudia Scalfi-Happ, Rudolf Steiner, Michael Beil

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500456

KEYWORDS: Luminescence, Laser scanners, Fluorescence lifetime imaging, Semiconductor lasers, Microscopes, Rhodamine, Time resolved spectroscopy, Picosecond phenomena, Microscopy, Photodynamic therapy

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SPIE Journal Paper | 1 January 2003 Open Access

Time-resolved microspectrofluorometry and fluorescence lifetime imaging of photosensitizers using picosecond pulsed diode lasers in laser scanning microscopes

Matthias Kress, Thomas Meier, Rudolf Steiner, Frank Dolp, Rainer Erdmann, Uwe Ortmann, Angelika Rueck

JBO, Vol. 8, Issue 01, (January 2003) https://doi.org/10.1117/12.10.1117/1.1528595

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This work describes the time-resolved fluorescence characteristics of two different photosensitizers in single cells, in detail mTHPC and 5-ALA induced PPIX, which are currently clinically used in photodynamic therapy. The fluorescence lifetime of the drugs was determined in the cells from time-gated spectra as well as single photon counting, using a picosecond pulsed diode laser for fluorescence excitation. The diode laser, which emits pulses at 398 nm with 70 ps full width at half maximum duration, was coupled to a confocal laser scanning microscope. For time-resolved spectroscopy a setup consisting of a Czerny Turner spectrometer and a MCP-gated and -intensified CCD camera was used. Time-gated spectra within the cells were acquired by placing the laser beam in ‘‘spot scan’’ mode. In addition, a time-correlated single photon counting module was used to determine the fluorescence lifetime from single spots and to record lifetime images. The fluorescence lifetime of mTHPC decreased from 7.5 to 5.5 ns during incubation from 1 to 6 h. This decrease was probably attributed to enhanced formation of aggregates during incubation. Fluorescence lifetime imaging showed that longer lifetimes were correlated with accumulation in the cytoplasm in the neighborhood of the cell nucleus, whereas shorter lifetimes were found in the outer cytoplasm. For cells that were incubated with 5-ALA, a fluorescence lifetime of 7.4 ns was found for PPIX; a shorter lifetime at 3.6 ns was probably attributed to photoproducts and aggregates of PPIX. In contrast from fluorescence intensity images alone, different fluorescence species could not be distinguished. However, in the lifetime image a structured fluorescence distribution in the cytoplasm was correlated with the longer lifetime and probably coincides with mitochondria. In conclusion, picosecond diode lasers coupled to a laser scanning microscope equipped with appropriate detection units allows time-resolved spectroscopy and lifetime imaging with high spatial resolution and provides numerous possibilities in cellular and pharmaceutical research.

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Proceedings Article | 2 November 2001 Paper

Short-pulsed diode lasers as an excitation source for time-resolved fluorescence applications and confocal laser scanning microscopy in PDT

Matthias Kress, Thomas Meier, Tarek El-Tayeb, Ralf Kemkemer, Rudolf Steiner, Angelika Rueck

Proceedings Volume 4431, (2001) https://doi.org/10.1117/12.447408

KEYWORDS: Luminescence, Semiconductor lasers, Time resolved spectroscopy, Laser scanners, Fluorescence spectroscopy, Spectroscopy, Microscopy, Photodynamic therapy, Argon ion lasers, Sensors

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Proceedings Article | 13 June 2001 Paper

Fluorescence method for monitoring of glucose in interstitial fluids

Maxim Loshchenov, Alexandr Parfenov, Gennady Kisselev, Alexander Stratonnikov, Katerina Ershova, Rudolf Steiner

Proceedings Volume 4263, (2001) https://doi.org/10.1117/12.429344

KEYWORDS: Glucose, Luminescence, Diagnostics, Spectroscopy, Laser scattering, Fiber optics tests, Fiber optics, Fluorescence spectroscopy, Sodium, Calibration

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Proceedings Article | 23 March 2001 Paper

Upconversion processes, luminescence kinetics, and cw diode-pumped 2.8-um laser action in Er-doped double-fluoride crystals

Alexandra Tkachuk, I. Mironov, V. Reiterov, Georgii Novikov, O. Orlov, Hans-Joachim Pohl, Rudolf Steiner, Martin Edelmann, V. Gapontsev

Proceedings Volume 4350, (2001) https://doi.org/10.1117/12.420948

KEYWORDS: Crystals, Luminescence, Erbium, Laser crystals, Upconversion, Semiconductor lasers, Continuous wave operation, Rod lasers, Pulsed laser operation, Energy transfer

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Proceedings Article | 16 November 2000 Paper

Simulation of the thermal effect of laser irradiation to optimize laser epilation

Detlef Russ, Alwin Kienle, Werner Falkenstein, Rudolf Steiner

Proceedings Volume 4161, (2000) https://doi.org/10.1117/12.409324

KEYWORDS: Tissues, Tissue optics, Alexandrite lasers, Laser tissue interaction, Absorption, Optical simulations, Nd:YAG lasers, Ruby lasers, Pulsed laser operation, Skin

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Proceedings Article | 2 July 1999 Paper

Time-resolved in-situ measurement of mitochondrial malfunction by energy transfer spectroscopy

Herbert Schneckenburger, Michael Gschwend, Wolfgang Strauss, Reinhard Sailer, Lars Schoch, Alexander Schuh, Karl Stock, Rudolf Steiner, Peter Zipfl

Proceedings Volume 3600, (1999) https://doi.org/10.1117/12.351027

KEYWORDS: Luminescence, Energy transfer, Signal detection, Light emitting diodes, Spectroscopy, Absorption, Fluorescence spectroscopy, Modulation, Pulsed laser operation, Time resolved spectroscopy

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Proceedings Article | 19 January 1999 Paper

Selective detection of mitochondrial malfunction in situ by energy transfer spectroscopy

Herbert Schneckenburger, Michael Gschwend, Reinhard Sailer, Wolfgang Strauss, Lars Schoch, Alexander Schuh, Karl Stock, Rudolf Steiner, Peter Zipfl

Proceedings Volume 3568, (1999) https://doi.org/10.1117/12.336827

KEYWORDS: Energy transfer, Luminescence, Absorption, Spectroscopy, Light emitting diodes, Signal detection, Rhodamine, Fluorescence spectroscopy, In situ metrology, Pulsed laser operation

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Proceedings Article | 4 January 1999 Paper

Spectral IR tissue diagnostics with photothermal detection

G. Schule, Bernhard Schmitz, Rudolf Steiner

Proceedings Volume 3565, (1999) https://doi.org/10.1117/12.335797

KEYWORDS: Tissues, Tissue optics, Diagnostics, FT-IR spectroscopy, Modulation, Skin, Polymethylmethacrylate, Absorption, Fused deposition modeling, Diffusion

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Proceedings Article | 4 January 1999 Paper

Spectral IR tissue diagnostics with photothermal detection

Georg Schuele, B. Schmitz, Rudolf Steiner

Proceedings Volume 3565, (1999) https://doi.org/10.1117/12.335794

KEYWORDS: Tissues, Tissue optics, Diagnostics, Skin, Fused deposition modeling, FT-IR spectroscopy, Polymethylmethacrylate, Absorption, Diffusion, Modulation

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Proceedings Article | 5 June 1998 Paper

3-um laser converter for medical applications

Rudolf Steiner, Hans-Joachim Pohl, I. Mironov

Proceedings Volume 3262, (1998) https://doi.org/10.1117/12.309481

KEYWORDS: Crystals, Holmium, Ions, Laser crystals, Er:YAG lasers, Laser applications, Ytterbium, Nd:YAG lasers, Laser therapeutics, Laser tissue interaction

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Proceedings Article | 19 May 1998 Paper

Confocal observation of hydrophilic and lipophilic photosensitizers in endothelial cells, lumen of the vessels, interstitium, and tumor cells using the chicken chorioallantoic membrane

Angelika Rueck, Nermin Akguen, K. Heckelsmiller, Gerd Beck, Felicitas Genze, Rudolf Steiner

Proceedings Volume 3247, (1998) https://doi.org/10.1117/12.308132

KEYWORDS: Tumors, Luminescence, Content addressable memory, In vivo imaging, Photodynamic therapy, Confocal microscopy, Microscopy, Tissues, Fluorescence spectroscopy, Blood vessels

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Proceedings Article | 1 January 1998 Paper

OCT images of human skin

Herman Brunner, Razvan Lazar, Ralf Seschek, Thomas Meier, Rudolf Steiner

Proceedings Volume 3194, (1998) https://doi.org/10.1117/12.301058

KEYWORDS: Optical coherence tomography, Skin, Tissue optics, Melanoma, Photomultipliers, Scattering media, Detector development, CCD cameras, Interferometry, Scattering

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Proceedings Article | 1 January 1998 Paper

3-um-laser converter for medical applications

Rudolf Steiner, Hans-Joachim Pohl, I. Mironov

Proceedings Volume 3199, (1998) https://doi.org/10.1117/12.301131

KEYWORDS: Crystals, Ions, Holmium, Laser crystals, Ytterbium, Laser tissue interaction, Laser applications, Nd:YAG lasers, Endoscopy, Quartz

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Proceedings Article | 1 January 1998 Paper

Fast OCT assembly using wide-area detection

Razvan Lazar, Herman Brunner, Ralf Seschek, Thomas Meier, Rudolf Steiner

Proceedings Volume 3194, (1998) https://doi.org/10.1117/12.301070

KEYWORDS: Optical coherence tomography, Sensors, Tomography, CCD cameras, Data acquisition, In vivo imaging, Head, Detector arrays, Detector development, Analog electronics

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Proceedings Article | 29 December 1997 Paper

Developing optimized tissue phantom systems for optical biopsies

Gerd Beck, Nermin Akguen, Angelika Rueck, Rudolf Steiner

Proceedings Volume 3197, (1997) https://doi.org/10.1117/12.297954

KEYWORDS: Particles, Scattering, Mie scattering, Absorption, Luminescence, Refractive index, Tissues, Tissue optics, Systems modeling, Light scattering

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Proceedings Article | 29 December 1997 Paper

Time-resolved energy transfer spectroscopy for measuring mitochondrial metabolism in living cells

Herbert Schneckenburger, Michael Gschwend, Wolfgang Strauss, Reinhard Sailer, Manfred Bauer, Rudolf Steiner

Proceedings Volume 3197, (1997) https://doi.org/10.1117/12.297964

KEYWORDS: Luminescence, Energy transfer, Spectroscopy, Rhodamine, Fluorescence spectroscopy, Control systems, Time resolved spectroscopy, Mode conditioning cables, Information operations, Mass attenuation coefficient

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