Multiphoton microscopy imaging of collagen fiber layers and orientation in the tympanic membrane

R. P. Jackson; C. Chlebicki; T. B. Krasieva; S. Puria

doi:10.1117/12.774353

8 February 2008 Multiphoton microscopy imaging of collagen fiber layers and orientation in the tympanic membrane

R. P. Jackson, C. Chlebicki, T. B. Krasieva, S. Puria

Proceedings Volume 6842, Photonic Therapeutics and Diagnostics IV; 68421D (2008) https://doi.org/10.1117/12.774353
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Greater understanding of tympanic membrane (TM) biomechanics has the potential to guide future advances in medical technology related to its surgical repair (myringoplasty). The pars tensa of the TM is a composite structure with two collagen fiber layers that provide the main scaffolding for the TM. The external layer is arranged in an approximately radial configuration, and the other is arranged in an approximately circumferential configuration. A more detailed knowledge of collagen fiber orientation and volume fraction could greatly improve existing mechanical simulations of the TM. To address this, we employed multiphoton microscopy (MPM) imaging of the TM in two modalities: second harmonic generation (SHG) and two-photon fluorescence (TPF). The unique spectral signature of SHG allows selective imaging of collagen fibers. TPF also produces images of fibrillar-type collagen but lacks the specificity of SHG. Both the SHG and TPF images show patterns of collagen organization in the TM that match expected results with respect to both orientation and size. Through MPM, we intend to accurately determine the collagen fiber layer thickness, density, and orientation as a function of radial position and quadrant location.

Citation Download Citation

R. P. Jackson, C. Chlebicki, T. B. Krasieva, and S. Puria "Multiphoton microscopy imaging of collagen fiber layers and orientation in the tympanic membrane", Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68421D (8 February 2008); https://doi.org/10.1117/12.774353

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