Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation

Dora Aït-Belkacem; Muriel Roche; Julien Duboisset; Patrick Ferrand; Sophie Brasselet; Marie Guilbert; Ganesh D. Sockalingum; Pierre Jeannesson

doi:10.1117/1.JBO.17.8.080506

14 August 2012 Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation

Dora Aït-Belkacem, Muriel Roche, Julien Duboisset, Patrick Ferrand, Sophie Brasselet, Marie Guilbert, Ganesh D. Sockalingum, Pierre Jeannesson

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Journal of Biomedical Optics, Vol. 17, Issue 8, 080506 (August 2012). https://doi.org/10.1117/1.JBO.17.8.080506

Abstract

Polarization resolved second harmonic generation (PSHG) is developed to study, at the microscopic scale, the impact of aging on the structure of type I collagen fibrils in two-dimensional coatings. A ribose-glycated collagen is also used to mimic tissue glycation usually described as an indicator of aging. PSHG images are analyzed using a generic approach of the molecular disorder information in collagen fibrils, revealing significant changes upon aging, with a direct correlation between molecular disorder and fibril diameters.

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Dora Aït-Belkacem, Muriel Roche, Julien Duboisset, Patrick Ferrand, Sophie Brasselet, Marie Guilbert, Ganesh D. Sockalingum, and Pierre Jeannesson "Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation," Journal of Biomedical Optics 17(8), 080506 (14 August 2012). https://doi.org/10.1117/1.JBO.17.8.080506

Published: 14 August 2012

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