Accounting for blood attenuation in intravascular near-infrared fluorescence-ultrasound imaging using a fluorophore-coated guidewire

Philipp Rauschendorfer; Georg Wissmeyer; Farouc A. Jaffer; Dimitris Gorpas; Vasilis Ntziachristos

doi:10.1117/1.JBO.28.4.046001

5 April 2023 Accounting for blood attenuation in intravascular near-infrared fluorescence-ultrasound imaging using a fluorophore-coated guidewire

Philipp Rauschendorfer, Georg Wissmeyer, Farouc A. Jaffer, Dimitris Gorpas, Vasilis Ntziachristos

Author Affiliations +

Journal of Biomedical Optics, Vol. 28, Issue 4, 046001 (April 2023). https://doi.org/10.1117/1.JBO.28.4.046001

Abstract

Significance

Intravascular near-infrared fluorescence (NIRF) imaging aims to improve the inspection of vascular pathology using fluorescent agents with specificity to vascular disease biomarkers. The method has been developed to operate in tandem with an anatomical modality, such as intravascular ultrasound (IVUS), and complements anatomical readings with pathophysiological contrast, enhancing the information obtained from the hybrid examination.

Aim

However, attenuation of NIRF signals by blood challenges NIRF quantification. We propose a new method for attenuation correction in NIRF intravascular imaging based on a fluorophore-coated guidewire that is used as a reference for the fluorescence measurement and provides a real-time measurement of blood attenuation during the NIRF examination.

Approach

We examine the performance of the method in a porcine coronary artery ex vivo and phantoms using a 3.2F NIRF-IVUS catheter.

Results

We demonstrate marked improvement over uncorrected signals of up to 4.5-fold and errors of <11 % for target signals acquired at distances up to 1 mm from the catheter system employed.

Conclusions

The method offers a potential means of improving the accuracy of intravascular NIRF imaging under in vivo conditions.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Philipp Rauschendorfer, Georg Wissmeyer, Farouc A. Jaffer, Dimitris Gorpas, and Vasilis Ntziachristos "Accounting for blood attenuation in intravascular near-infrared fluorescence-ultrasound imaging using a fluorophore-coated guidewire," Journal of Biomedical Optics 28(4), 046001 (5 April 2023). https://doi.org/10.1117/1.JBO.28.4.046001

Received: 14 November 2022; Accepted: 20 March 2023; Published: 5 April 2023

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